Lab Practical 2 - Sheet1 (1).csv

Gram negative rods. Oxidase negative. Catalase positive. Facultative anaerobes. Ferment glucose. Usually cause GI diseases. Main difference is the ability to ferment Lacotose or Non lactose fermenting. Lac positive are usually normal flora and cause opportunistic infections nosocomial. Where as lac negative are normally outright pathogens.

Cannot ferment sorbital. Main difference between normal Escherichia coli. this can be distinguished by a SMAC plate. If the E.coli does not ferment the sorbital in the plate it is O156H7

Isolation of most Salmonella species. Selective agents. Brilliant green inhibits growth of Gram positives and most Gram negatives highly selective for Salmonella. DIfferential agents Lactose and sucrose bacteria that ferment these sugars produce yellowish colonies with yellow halo. Salmonella spp. do not ferment lac or suc colonies appear white with red halo

"Primary plating medium for Gram negative enterics. Selective agents Eosin & methylene blue dyes: select for growth of Gram negatives

"Primary plating medium for Gram negative enterics. Selective agents Bile salts inhibit growth of many Gram positives and non-enteric Gram negatives. Selective agents Sugars (lactose sucrose salicin): bacteria that ferment 1 or more of these sugars produce yellow/orange colonies

Primary plating medium for Gram negative enterics. Selective agents Bile salts and crystal violet inhibit growth of most Gram positives. Differential agents. Lactose: bacteria that ferment lactose produce bright pink colonies. E. coli (and some other enterics) precipitate bile salts and produce cloudy zones around the colonies

Isolation and identification of E. coli O157H7. Exactly same as MAC plate but has Sorbitol bacteria that ferment sorbitol produce bright pink colonies. Since O157H7 can not ferment this it will have buff colored colonies.

"Isolation and identification of Salmonella and Shigella species. Selective agents Biles salts and various dyes inhibit growth of most Gram positives.Differential agents Lactose bacteria that ferment lactose produce reddish colonies. H2S production bacteria that produce H2S form colonies with black centers. Salmonella and Shigella will form colorless colonies Salmonella is positive for H2S

"Glucose lactose and sucrose fermentation pattern Ferrous iron forms black precipitate if H2S is produced. Yellow butt red slant only glucose fermented.Yellow butt yellow slant glucose and lactose OR sucrose fermented

"Primary plating medium for Gram negative enterics especially useful for Shigella isolation/identification. Selective agent Deoxycholate (bile) inhibits most Gram positives. Selective agents Xylose bacteria that ferment xylose produce yellow coloniesH2S production bacteria that produce H2S form colonies with black centers. Shigella does not ferment xylose and appears red Salmonella ferments xylose and appears yellow

Used primarily for Lac negative Enterobacteriacae. Are the sugars Lysine. Orithine. Arnthenine. and a Control. Must use mineral oil on top to create anaerobic environment for fermentation. Works by removing carboxylic acids and can use them for an energy source making the solution basic. The tubes contain glucose plus one of the amino acids. If the bacteria can use the AA the solution will turn basic by breaking down the AA to an amine. The solution will turn back to its original blue color. yellow is a negative test.

Is an enzyme that removes an amine from phenylalanine. Uses teh enzyme deaminase to create phenyl pyruvic acid and an amine. To test this add ferric choloride to the slant and look for a BRIGHT GREEN COLOR.

TSI A/AG-. h2s negative. IDOLE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. vp negative. citrate negative. urease negative. maltose negative.

TSI A/AG-. h2s negative. IDOLE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. vp negative. citrate negative. urease positive. maltose negative. Main test SMAC PLATE WITH DULL COLORED COLINES. Not orange like normal e. coli.

A/AG -. ((H2S POSITIVE)). Indole negative. MOTILITY POSITVE. . MR POSITIVE. vp negative. ((CITRATE POSITIVE)). . UREASE POSITVE. ((MALONATE NEGATIVE)).

A/AG-. h2s negative. INDOLE POSITIVE. Motility positive. MR POSITIVE. . vp negative. CITRATE POSITIVE. UREASE POSITVE ((MALONATE POSITIVE). Between fruendi and diverusus use malonate and h2s and indole.

A/AG -. h2s negative. indole negative. MOTILITY POSITVE. mr negative. VP POSITVE. CITRATE POSITVE. urease negative. MALONATEPOSITIVE. VERY MOTILE!!!!

A/AG-. h2s negative. Indole negative. motility negative. mr negative. VP POSITIVE. UREASE POSITVE. CITRATE POSITVE. MALONATE POSITVE. to differentiate between citrobacter and klebisella use molilty and that citrobacter is MR positve. Remeber k. pneumonia is different from k. oxytoca by indole

A/AG-. h2s negative. ((INDOLE POSITIVE)). motility negative. mr negative. VP POSITIVE. UREASE POSITVE. CITRATE POSITVE. MALONATE POSITVE. to differentiate between citrobacter and klebisella use molilty and that citrobacter is MR positve. Remeber k. pneumonia is different from k. oxytoca by indole

Eserichia coli. Citrobacter deversus. Citrobacter freundii. Enterobacter areogenes. Klebisella pneumonia. Klebisella oxytoca.

SWARMER. K/AG+.(( H2S NEGATIVE)) SULFIDE POSITIVE. MOTILITY POSITVE. MR POSITIVE. vp negative. CITRATE POSITVE. UREASE POSITVE. PHEN. DEAMINASE POSITVE. Lysine decarb negative. arginine decarb negative. Ornithine decarb. Sucrose positve

SWARMER (((((A/A+))))). INDOLE POSITIVE. SULFIDE POSITIVE. MOLTILITY POSITIVE. MR POSITIVE. citrate negative. UREASE POSITIVE. . PHEN DEAMINASE POSITVE. Lysine decarb. Arginine decarb negative. Orithine negative. SUCROSE POSITIVE.

K/A-. INDOLE POSITIVE. (((SULFIDE NEGATIVE)))). MOTILITY POSITIVE. MR POSITIVE. vp negative. CITRATE POSITIVE. UREASE NEGATIVE. PHENYLDECARB POSITVE. lysine. ariginine ornithine negative. sucrose negative

PROTEUS IS A SWARMER AND HIGHLY MOTILE. ALL PHEN. DEAMINASE POSITIVE. MOTILITY POSITIVE. Proteus stuartii is the only H2S NEGATIVE. Proteus mirabolis is K/AG+ and indole and sucrose negative but citrate postive ornithine Positve. Proteus vulgaris is A/Ag+ and ferments sucrose. Is indole negative and cirtrate negative. ornithine negative.

K/AG-. indole negative. sulfide negative. motility negative. MR POSITVE. vp negative.urease negative. Phen. deaminase negative. lysine decarb. negative. ARGININE DECARB POSITVE. Orithine negative. Sucrose negative

K/AG-. indole negative. sulfide negative. motility negative. MR POSITVE. vp negative.urease negative. Phen. deaminase negative. lysine decarb. negative. arginine decarb negative.. ORINTHINE POSITVE. Sucrose negative

K/AG+. Indole negative. SUFLIDE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. CITRATE POSITIVE. vp negative. Phenyl deaminase negative. LYSINE POSITVE ARGININE POSITIVE. ORNITHINE POSITVE. Sucrose negative

A/A- INDOLE POSITIVE. Sulfide negative. MOTILITY POSITIVE. MR POSITIVE. VP negative. Citrate negative. UREASE POSITIVE. Phen. deaminase negative. Lysine decarb negative. Arginine decarb negative. ORNITHINE POSITIVE. SUCROSE POSITIVE. IS NON MOTILE AT 35 DEGREE BUT MOTILE AT ROOM TEMP.

If it is PHEN. DEAMINASE POSITVE AND MOTILE (SWARMING) PROTEUS.. Negative everything except MR or One decarb AA is SHIGELLA. ALL POSITVE DECARBS IS SALMONELLA TYPHIMURIUM. If it is H2S negative and Indole positve and phen. negative but sucrose postive is Yersina enterocolitica.

Look for H2S production. Non H2S is Y. stuartii. Only H2S positive with A/AG+ is P. vulgaris and this is because of SUCROSE. P. mirabilis is positive for citrate and negative indole and positive ornithine.

Gram negative s shaped. can be helical. OXIDASE POSITIVE. Microaerohilic. When staining it is hard to do secondary stain and must use carbofusion instead of safranin.

Isolation and growth of Campylobacter especially C. jejuni. Selective agents Antibiotics (cefoperazone vancomycin amphotericin B) inhibit growth of other bacteria. Contains sheep blood

Isolation and growth of Campylocbacter species. Antibiotics (cefoperazone vancomycin cyclohexamide) inhibit growth of other bacteria. Blood-free medium charcoal acts as a quencher of reactive O2 species

Isolation and enumeration of marine bacteria. selective High salt concentration: selects for halophilic bacteria. No differential agent Vibrio cholerae is not halophilic and will not grow! All other Vibrio species should grow

"Isolation and identification of Aeromonas species. Maltose colonies that ferment maltose appear yellow. Thiosulfate detection of H2S production. Contains novobiocin. Lysine and ornithine bacteria that decarboxylate lysine or ornithine turn media yellow.

"Primary isolation of Vibrio species particularly V. cholerae. Bile salts inhibit Gram positive organisms

NALIDIXIC ACID SENSITIVE. (disk placed on campy CVA agar) cephalothin resistant (disk on campy CVA). GROWTH AT 42 POSITIVE. HIPPURATE POSITIVE.

Nalidixic acid negative. CEPHALOTHIN SENSITIVE. Growth at 42 negative. hippurate negative

Chemical test that use the indicator dye phenyl red to indicate if fermentation of these sugars can be conducted. Test used for vibrio species

Yellow colonies on TCBS agar (sucrose ferm). BETA hemolysis. SENSITIVE TO O129 disk. GROWTH AT 0 NACL. GROWTH AT 1 NACL. GROWTH AT 3 PERCENT NACL. No growth from 6-10 NaCL. Lysine positivite. ORINTHINE POSITIVE. pr arabinose negative. PR GALACTOSE POSITIVE. Urease negative.

Light blue or green colonies on TCBS agar (no sucrose ferm). Alpha hemolysis. NO GROWTH AT 0 NACL. GROWTH FROM 1 TO 6 PERCENT NACL. No growth at 10 percent bile. ORNITHINE POSITIVE. PR ARABINOSE PR GALACTOSE. ((((UREASE POSITIVE)))

Yellow colonies (sucorse ferm on TCBS). Alpha beta hemolysis. O129 SENSITIVE. No growth at 0 NaCl. GROWTH FROM 1 TO 6 PERCENT NACL. No growth at 10 percent. ORINTHINE NEGATIVE. Arginine negative. Urease negative. Similar to V. cholera but can not grow without NaCl.

Yellow colonies on TCBS. O129S. No growth at 0 NaCl. GROWTH FROM 1 TO 10 PERCENT NACL. HALOPHILE. NEGATIVE ORNITHINE. NEGATIVE ARGININE. NEGATIVE PR ARABINOSE AND PR GALACTOSE. Negative urease.

If growth on TCBS will be blue colonies. YELLOW COLONIES WITH BLACK CENTERS (H2S) ON RM (rimler shots media maltose fermentation. thiosulfate). (((O129 RESISTANT))). GROWTH FROM 0 TO 3 PERCENT NACL. No growth from 6 to 10 percent. LYSINE POSITIVE. Ornithine negative. ARGININE POSITVE. PR ARABINOSE AND PR GALACTOSE POSTIVE. Urease negative

For Vibrio cholera use the growth at 0 percent NaCl. With the beta hemolysis and growth on TCBS media. For Vibrio parahemolyticus you can use the blue colonies on TCBS media and with its ability to be urease positve and additionally it is both PR arabinose and galactose positive. For Vibrio vulnificus you can look at the no growth at 0 percent NaCl along with growth up to 6 percent NaCl. For Vibrio alginolyticus you can use the fact it is a halophile and will grow up to 10 percent NaCl also it does not use both arabinose and galactose.

Use the fact that Vibrio is sensitive to O129 disks where Aermonas is not. Aeromonas will grow on RS media which will show yellow colonies with H2S production along with maltose fermentation. Aeromonas is additonally arabinose and galactose positive.

Mainly intestional pathogens. A comma shaped bacteria that is gram negative and OXIDASE POSITIVE. Most need NaCl to grow except for cholera.

HAVE OXIDATIVE METABOLISM. Strict aerobes unless you give a different final acceptor like Nitrate. TSI/ IS K/N-. Found in normal flora. solil. water. associated with plants. can cause wound infections. Bacteria are pseudomonas aeruginosa. pseudomonas fluorescens. Acinetobacter baumanii. Alcaligenese faecalis. Stenotrophomonas maltophilia. Shewanella putrifaceins.

Selective isolation of Pseudomonas aeruginosa. Selective agents Cetrimide a cationic surfactant that inhibits growth of normal flora. Differential agents Cetrimide enhances pigment production by P. aeruginosa. Pigments produced by P. aeruginosa include pyocyanin (blue-green) and fluorescein (green-yellow)

Stimulates production of fluorescein pigment and inhibits production of pyocyanin pigment. Differential agent Magnesium sulfate: stimulates fluorescein production. Pseudomonas aeruginosa appears yellow-green due to fluorescein production

Stimulates production of pyocyanin pigment and inhibits production of fluorescein pigment. Magnesium chloride and potassium sulfate stimulate pyocyanin production. Pseudomonas species that produce pyocyanin will form blue pigment

Oxidase positive. Beta hemolysis. TSI K/N-. P AGAR POSITIVE. F AGAR POSITIVE. NITRATE REDUCTION (N2). UREASE POSITIVE. GLUCONATE POSITIVE. GELATIN HYDROLYSIS. O/F GLUCOSE. O/F MALTOSE. Growth at 5 celciuss. GROWTH AT 42 POSITIVE. Lac negative on MAC. GROWTH ON CET GREEN.

Oxidase positive. Weak beta hemolysis. K/N- P agar negative. F AGAR POSITIVE. Nitrate reduction negative. UREASE POSITVE. GLUCONATE POSITVE. MOTILITY POSITIVE. GELATIN HYDROLYSIS. O/F GLUCOSE POSITIVE. O/F maltose negative. (((GROWTH AT 5 DEGREES))). No growth 42 degree. Lac - MAC. Growth on CET white colonies..

OXIDASE NEGATIVE. Weakly beta hemolytic. K/N-. P agar negative. F agar negative. Nitrate negative. urease negative. gluconate negative. motility negative. gelatin hydrolysis negative. o/f glucose negative. o/f maltose negative. growth at 5 degree negative. GROWTH AT 42 degree. GROWTH ON MAC PLATE WITH SLIGHT PINK COLONIES Growth on CET white colonies.

Oxidase positive. K/N- . P agar negative. F agar negative. Nitrate reduction negative. urease negative. glucontate negative. ((MOTILITY POSITIVE)). gelatin hydrolysis negative. o/f glucose negative. o/f maltose negative. growth at 5 degree negative. growth at 42 growth positive. apperance on MAC lac negative. Growth on CET white colonies.

OXIDASE NEGATIVE. K/N-. P agar negative. F agar negative. (((NITRATE REDUCTION POSITIVE))). UREASE POSITIVE. gluconate negative. MOTILITY POSITVE. GELATIN HYDROLYSIS POSITIVE. O/F GLUCOSE POSTIVE. (((O/F MALTOSE))). no growth at 5 or 42 degree. Apperance on MAC lac -. Growth on CET white colonies.

Oxidase positive. gamma hemolysis. (((K/N+))). SULFIDE POSITVE. p agar negative. f agar negative. NITRATE REDUCTION POSITIVE (N2). UREASE POSITVE. Gluconate negative. MOTILITY POSITVE. GELATIN HYDROLYSIS POSITIVE. O/F glucose negative. O/Fmaltose negative. No growth at 5 or 42 degree. Mac plate is G-. ((NO GROWTH ON CET PLATE)).

Gram negative cocci in pairs. Diplococci. Can be kidney shaped. N. mennigitis and N. gonorrhea are fastidious and only survive in humans and 48 hours in lab media. Grow in increased CO2. OXIDASE POSITIVE. all aerobic. Specific ones N. gonorrheae. N. meningitidis. N. sicca. N. muscosa. and Moraxella catarrhalis which is a coccobacillus that has similarities to neisseria. OXIDATIVE ATTACK OF SUGARS!! USE CTA SUGARS TO CHECK FOR OXIDATION

Chocolate agar base but has antibiotics (vancomyocin. cholistin. and nistatin in to inhibit other bacterial growth).

GROWTH ON MTM POSITIVE. Growth on TSA (fastidious). CTA GLUCOSE POSITIVE. cta sucose. lactose. maltose. fructose. negative. Dnase negative. nitrate reduction negative. nitrite reduction.

GROWTH ON MTM POSITVE. Growth on TSA. CTA GLUCOSE AND MALTOSE POSITIVE. cta sucrose. lactose. fructose. dnase negative nitrate reduction negative. ntirite reduction negative.

growth on mtm negative. GROWTH ON TSA. CTA GLUCOSE. SUCOSE MALTOSE. FRUCTOSE POSITIVE. cta lactose negative. dnase negative. (((NITRATE REDUCTION POSITIVE))) NITRITE REDUCTION POSITIVE.

growth on mtM. GROWTH ON TSA. CTA GLUCOSE. SUCROSE. MALTOSE. FRUCTOSE. POSITIVE. cta lactose negative. dnase negative. nitrate reduction negative. NITRITE REDUCTION POSTIVE.

Growth on mTM variable. GROWTH ON TSA. ((ALL CTA NEGATIVE. glucose. sucrose. fructose. lactose. maltose))). DNASE POSITIVE. NITRATE REDUCTION. NITRITE REDUCTION.

Blood loving bacterai. Gram negative coccobacillus. Grow with increased CO2. Fastidious. REQUIRMENT ARE CO FACTORS.

Heme.

NAD (positve)

X factor requried. Do not require heme will be positive. No substrate. ALA substrate porphobilinogen precursor of protoporphyrin which will turn to heme. Detects porphyrin UV light.

Porphyrin synthesis negative. CAS POSITIVE. X negative. V negative. CHOCOLATE POSTIVE. TSA Plate no growth X only or Y only (X+Y GROWTH)

PRORPHYIN SYNTHESIS POSITIVE. CAS +. x negative. V POSITIVE. CHOCOLATE POSITVE. TSA plate x negative (V AND X+V POSITIVE.) NEEDS V ONLY!!!!!

Has 4 quadrants. I has BA which is x/y used for hemolysis. II is x only. III is V only. IV is chocolate agar used as a control.

Will streak a BA plate with S. aureus to cause hemolyosis and release X cofactor. Resease of heme.

pre reduced anaerobically sterilized media. Prepared and stored under oxygen free conditions.

Isolation and differntiatino of anaerobes particular Bacteroides. Has Gentamiicin inhibits facultative anaerobes. Bile selects for resistant anaerobes such as Bacteroides. DIFFERENTIAL AGENTS. Esculin hydrolysis of esculin produces blackening of medium around colonies.

Brucella BA. Used for isolation and cultivation of obilgate anaerobes from clincial specimens. Sheep blood for hemolysis reaciton. Non selective media. general purpose.

Brucella leaked vancomycin and kanamycin. Used for identification of obiligately anaerobic Gram negative bacilli. Selective agents are vancomycin and kanamycin. DIFFERENTIAL AGENT vitiamin K1 which enhances pigment production by Prevotella. Blood is laked or lysed to release heme.

Used for the isolation and identification of Clostridium difficile. Selective agents are cefoxitin and cycloserine inhibt most fecal flora. DIFFERENTIAL. fructose adn ph indictator. If fructose fermenetatin occurs media will turn yellow that fluoresce golden under UV light.

Cultivation and isolation of anaerobes. NO selective agents. DIFFERENTIAL AGENTS. egg yolk. lecithinase will appear as opaque precipitate around coloniesl Lipase iridescent sheen on colonies. Proteolysis clearing of medium around colonies.

cultivation and isolation of anaerobes. Phenylethyl alcohol inhibits gorwth of gram negative facultatively anaerobic bacteria. prevents swarming of proteus. NO differential agents. Useful for isolation of anaerboes from mixed culturs taht might contain Proteus.

Growth of anaerobes no selective agents. DIFFerntial agent is sodium thioglycollate creates oxygen gradient from top to bottom of tube. Obligate anaerobes will grow at the bottom where there is no oxygen.

Gamma hemolytic. Lecithiniase negative. LIPASE POSITIVE. Disk sensitivity Colistin resistant Kanamyicin Sensitive Vancomycin Sensitive. (RSS) NO LITMUS MILK REACTION

Negative lecithinase. Negative lipase. POSITIVE PROTEASE. Weak beta. LITMUS MILK CURD. Semisolid from curd production from coagulation of caesin. Disks Colistin Resistant. kanamycin Sensitive. Vancomycin Sensitive. (RSS)

Swarming growth. Lecithiniase negative. Lipase negative. Protease negative. Disk senstivity Colistin Resistant. Kanamycin Sensitive. Vancomycin Sensitive. stormy fermentation

Gamma Lecithinase negative. Lipase negative. Protease negative. Disk appearance Colistin Resistant. Kanamycin Sensitive. Vancomycin Senstive (RSS). LITMUS MILK REACTION TO P. DIGESTION OF PEPTONES. CLEAR MEDIUM PEPTONIZATION

Bru BAP double zone of hemolysis. LECITHINASE POSITIVE. Lipase negative. Protease negative. Disk sensitivity Colistin Resistant. Kanamycin Sensitve. Vancomycin Sensitive. LITMUS MILK IS STORMY FERMINATION. CLOT BROKEN APART.

gram negative. bacillis KANAMYCIN COLISTIN VANCOMYCIN RESISTANT. CAN GROW IN 20 PERCENT BILE.

Gram negative bacillus. Kanamycin resistant. VANCOMYCIN SUSCEPTIBL (is normally used on Gram positive) Resistant to kanamycin and colistin which are normally for G negative) E. EXTRA TEST PIGMENT!!!!

Gram negative KANAMYCIN RESISTANT. VANCOMYCIN RESISTANT. COLISTIN SUSCEPTIBLE. (((RED FLUORENCE)))

Gram negative bacillus. KANAMYCIN SENSITIVE. VANCOMYCIN REISTANT. COLISTIN SENSITIVE. (((GROWTH 20 PERCENT BILE)))\\

gram negative bacillus kanamycin sensitive. vancomycin resistatn. colistin sensitive. NO BILE GROWTH

SPS SENSITIVE. gram positive cocci. kanamycin resistant. Vancomycin Susecptible. Colistin resistant

Gram positive bacillus

Used for the isolation of Legionella pneumophila. Aerosolized in hot tub. Lung pathogen

Will grow well on MAC plate. UREASE POSITIVE STRONG. INDOLE POSITIVE.

DOES NOT GROW ON MAC. INDOLE NEGATIVE. Nitrate reduction positive. Aerobic oxidation