- the nucleotide sequence in a mRNA molecule specifies the amino acid sequence of a protein. Ribosomes in the cytoplasm carry out translation.
- 1) An mRNA molecule binds to the small ribosomal subunit at the binding site. A special tRNA called the intiator binds to start the codon on mRNA, where translation begins.
2) next the large ribosomal subunit attaches with the small ribosomal subunit creating a functional ribosome.
3) the anticodon of another tRNA with its attached amino acid pairs with a second mRNA codon at A site of the ribosome
4)A component of the large ribosomal subunit catalyzes the formation of a peptide bond between methionine, which seperates from the tRNA at the P site , and the amino acid carried by the tRNA at the A site.
5) tRNA at the P site detaches, and the ribosome shifts the mRNA strand by one codon. The tRNA in the A site moves to the P site allowing another tRNA with its amino acid to bind to a newly exposed codon at the A site. steps 3 through five repeat
6) protein synthesis ends when the ribosome reaches a stop codon at the A site which causes the completed protein to detach from the final tRNA