Microbio Midterm 1

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Author:
Hatsumi743
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158826
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Microbio Midterm 1
Updated:
2012-06-15 00:00:29
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Microbio
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Microbio Midterm 1 Questions
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  1. Early metabolism may have been catalyzed by _____
    RNA
  2. The 1st person to visualize individual microbes was...
    Antoni Van Leeuwenhoek
  3. The use of agar as the gelling agent in solid media was suggested by whom?
    Angela Hesse
  4. The genetic expression machinery of archaea is most similar to _____
    Eukaryotes
  5. Which of the folloing staining processes requires crystal violet?
    Gram Staining
  6. 2D polyacrylamide gel electrophoresis involves all of these except what? 

    A) DNA sequencing
    B) Sodium dodecyl sulfate (SDS)
    C) Staining for detection
    D) electrophoresis
    E) isoelectric focusing
    A) DNA sequencing
    (this multiple choice question has been scrambled)
  7. The chromosome includes a number of extra twists called supercoils. All of the following are true of supercoiling except what?

    A) DNA doubles back and twists upon itself
    B) It is generated by gyrase
    C) In bacteria and eukaryotic cells, there is positive supercoiling
    D) It is maintined by DNA-binding proteins.
    E) This results in compaction
    C) In bacteria and eukaryotic cells, there is positive supercoiling
    (this multiple choice question has been scrambled)
  8. Which name refers to having flagella attached at one or both ends of the cell?
    Iophotrichous
  9. In the lab, E. coli can divide into 2 cells every _____
    20-30 mins
  10. Which is a micronutrient?

    A) carbon
    B) calcium
    C) nitrogen
    D) zinc 
    E) sulfur
    D) zinc
    (this multiple choice question has been scrambled)
  11. All of the following are true about MacConkey medium except what?

    A) it is both selective and differential
    B) it is selective
    C) Lactose fermenters appear colorless 
    D) It contains bile salts
    E) it is differential
    C) Lactose fermenters appear colorless
    (this multiple choice question has been scrambled)
  12. Which of the following can be used to assess protein expression under different culture conditions?

    A) PCR 
    B) DNA microarrays
    C) 2D protein gels
    D) viable count
    E) turbidity
    C) 2D protein gels
    (this multiple choice question has been scrambled)
  13. Okazaki fragments are approx. how many bases in length?
    1000
  14. Which molecule is responsible for removing torsional stress during DNA replication?
     
    DNA gyrase
  15. The eukaryotic predators of the microscopic world are ____
    Protists
  16. Which of the following is not a palindrome?

    A) toot
    B) rotor
    C) racecar
    D) radar
    E) toto 
    E) toto
    (this multiple choice question has been scrambled)
  17. Which of the following is not rue about the 2 strands of DNA in a double helix?

    A) antiparallel
    B) base-pairing interactions
    C) base-stacking interactions 
    D) complementary
    E) catenated
    E) catenated
    (this multiple choice question has been scrambled)
  18. Which of the following is not part of initiation of transcription?

    A) the RNA polymerase holoenzyme
    B) base pairing of the 1st nucleotide of the RNA 
    C) synthesis of a primer
    D) binding of RNA polymerase to the beginning of the gene
    E) melting of the helix
    C) synthesis of a primer
    (this multiple choice question has been scrambled)
  19. The energy souce utilized by the elongation factors EF-G and EF-Tu is ____
    GTP hydrolysis
  20. Post translational modification may involve

    A) adenylation
    B) phosphorylation
    C) acetylation
    D) all of the above
    E) none of the above 
    D) all of the above
  21. Specific membrane components, particularly ________, determine which substances are transported across the membrane.
    Proteins
  22. Evolution toward pathogenicity may involve _____

    A) horizontal gene transfer
    B) gene loss
    C) gene acqusition
    D) A and C
    E) all of the above 
    E) all of the above
  23. Hydrolysis of ATP with the release of pyrophosphate is the driving force of which of the following reactions?

    A) DNA synthesis
    B) RNA synthesis
    C) aminoacylation of tRNA
    D) all of the above
    E) none of the above 
    D) all of the above
  24. In glycolysis, dihydroxyacetone phosphate is converted to ____
    Glyceraldehyde-3-phosphate
  25. T or F: Antiseptics and antibiotics are both used orally to fight bacterial infections
    F
  26. T or F: The bacterial nucleoid is bound by a membrane
    F
  27. T or F: During biofilm formation, as more and more cells bind to the surface, they begin to send signals to each other in a process called quorum sensing 
    T
  28. T or F: Extremophiles typically evolve to survive multiple extreme envirionments
    T
  29. T or F: The ribosome has no enzymatic activity
    F
  30. T or F: Only the nuclotide triphosphate ATP carries energy
    F

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