Microbio Midterm 2

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  1. Which epidemic is not virus borne?

    A) bubonic plague
    B) smallpox 
    C) AIDS
    D) SARS
    E) polio
    A) bubonic plague
    (this multiple choice question has been scrambled)
  2. An icosahedral capsid has how many sides?
    20
  3. Viruses may be classified by the _______ method
    Baltimore
  4. So far, the known mechanisms of replication and mRNA expression define how many fundamental groups of virus species?
    7
  5. The period of time when virions are virtually undetectable inside the infected cell is the ______
    Eclipse Period
  6. Which form of control is the least reversible and most drastic?

    A) control of post-transcripton
    B) control of transcription
    C) posttranslational control 
    D) alterations of DNA sequence
    E) translational control
    D) alterations of DNA sequence
    (this multiple choice question has been scrambled)
  7. Which is the favored carbon source of E. coli? 
    Glucose
  8. Transcriptional attenuation is a common regulatory strategy used to control many operons that code for what?
    Amino acid biosynthesis
  9. Which of the following is not true of phase variation?

    A) The orientation of the DNA sequence is inverted
    B) It only occurs in prokaryotes
    C) All are true 
    D) It may help the bacteria avoid the immune system
    E) It can result in a change in microbial appearance
    B) it only occurs in prokaryotes
    (this multiple choice question has been scrambled)
  10. Integrated circuits can:

    A) send a virus down alt. lifestyle paths
    B) couple the genetic and biochemical control of a metabolic pathway
    C) time the envents of a developmental cycle
    D) all of the above
    E) none of the above 
    E) all of the above
  11. Which of the following is a useful reporter gene?

    A) X gal
    B) lacZ 
    C) horseradish peroxidase
    D) gadA
    E) transposase
    B) lacZ
    (this multiple choice question has been scrambled)
  12. At what level can a cell regulate production of a protein?

    A) transcription
    B) translation
    C) stability of the mRNA
    D) all of the above
    E) none of the above 
    D) all of the above
  13. Which of the following statements is false with respect to reporter fusions?

    A) reporter genes encode proteins whose activity is easily assayed
    B) gene regulation can be examined by fusing the promoter of the gene to a reporter gene
    C) all are true
    D) reporter fusions facillitate monitoring the expression of genes whose products are difficult to measure directly
    E) all are false 
    C) all are true
    (this multiple choice question has been scrambled)
  14. Which of the following techniques would be used to monitor changes in mRNA levels?

    A) SDS-PAGE 
    B) Southern blots
    C) Northern blots
    D) Eastern blots
    E) Western blots
    C) Northern blot
    (this multiple choice question has been scrambled)
  15. Which technique would be used to monitor changes in protein levels?
    Western blot
  16. In the western blot procedure, proteins are transferred from the gel to a ____ 
    Polyvinylidine fluoride (PVDF) membrane
  17. The organism producing an insecticidal protein is _____
    Bacillus thurigiensis
  18. Which metabolism is thought to be absent in the 1st cells?
    Aerobic Respiration
  19. Which is correct nomenclature of ESCHERICHIA COLI?
    Escherichia coli
  20. The description of distinct life forms and their organization into different categories is called ______
    Taxonomy
  21. Which of the following would not be a good choice to use as a molecular clock to compare the 3 domains?

    A) tRNA
    B) rRNA
    C) RNA polymerase
    D) chloroplast proteins 
    E) ribosomal proteins
    D) chloroplast proteins
    (this multiple choice question has been scrambled)
  22. Which of the following scientists is responsible for defining the Archaea as a 3rd domain?

    A) Rosalind Franklin 
    B) Francis Crick
    C) Carl Woese
    D) James Watson
    E) Norm Pace
    C) Carl Woese
    (this multiple choice question has been scrambled)
  23. Which of the following does not typically contribute to horizontal gene transfer?

    A) genome replication
    B) bacteriophages 
    C) transposable elements
    D) viruses
    E) plasmids
    A) genome replication
    (this multiple choice question has been scrambled)
  24. Fungi grow hyphae with cell walls of _____
    Chitin
  25. Sets of genes in operons are coordinately regulated by which of the following?

    A) protein repressors
    B) activators
    C) sigma factors
    D) small RNAs
    E) all of the above 
    E) all of the above
  26. If a gene is always expressed, it is ______
    Constitutive
  27. Which phyla produces what are called "true" mushrooms?
    Basidiomycetes
  28. T or F: All 3 gene products of the lac operon are required for the utilization of lactose to occur
    F
  29. T or F: Fossil stromatolites are among the earliest forms of life on Earth
    T
  30. T or F: Some yeasts can undergoes alt. of generations
    T
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Microbio Midterm 2
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Microbio Midterm 2 Questions
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