Card Set Information
NO CELL WALL
Need complex media
Mycoplasmas adhere to the epithelium of mucosal surfaces
Mycoplasma Class Mollicutes
What are the 3 species of Human pathogens for Mycoplasma?
M. pneumoniae (Respiratory)
M. hominis (genital)
How are the Human pathogens for Mycoplasmas transmitted?
Mother to child
How do you collect a specimen for Mycoplasmas?
No cell wall so are susceptible to drying
Immediate transport to lab is important
Swabs are placed with TSB 0.5% albumin and 400U penicillin
Specimen should be placed at -70C if not processed immediately
What are the growth conditions for Mycoplasma?
Cultures are seldom ordered
Require trained personal (experts)
Only 40% sensitivity
: SP4 broth/agar and 5-10% CO2
What organism requires only SBA and arginine?
What organism requires SBA and urea and can also use Shepherd's 10 B Broth?
What are more growth conditions required for Mycoplasma?
Blood Cultures need 1% gelatin to decrease toxicity of SPS
Collection must be ASAP (due to dryness)
Dracon and Ca alginate swabs with metal shaft
TSB with 0.5% albumin and 400U/mL of penicillin for transport
How do you identify Mycoplasmas?
Like colonies stained with Dienes stain
Colonies have a typical fried egg appearance
Stained colonies have a light periphery and dark-blue center
How long does M. hominis take to grow?
How long does M. pneumoniae take to grow?
What organisms grow in NY city agar as minute colonies, only observed with a stereoscope?
What are the Serological procedures to identify Mycoplasmas?
Cold agglutinins (used for detecting atypical penumonia)
EIA and IHA detect both IgG and IgM
PCR (very specific)
Reference labs perform serology on what organisms?
Characteristics of Mycoplasma pneumoniae are:
Causes a typical pneumonia or "walking pneumonia"
20% of the reported pneumonias
Higher incidence in young adults (not seasonal)
Groups at risk
: college students, military
Transmission is by aerosol droplets
Most infections asymptomatic or mild
Incubation period 2-3 weeks
: sore throat, dry cough, fever, malaise, anorexia
Extrapulmonary infections are rare
Characteristics of M. hominis and Ureaplasma urealyticum:
Associated with urogenital tract
Higher rates in Africans (men and woman)
M. hominis found in 50% of healthy adults (interpretation difficult when asymptomatic)
Reported as cause of Non gonococcal urethritis
What organism causes salpingitis, pelvic, inflamatory disease, and post-partum fever?
What organism causes 10% of cases of Non gonococcal urethritis in man and upper female genitourinary tract disorders?
What are the antimicrobials these organisms are susceptible?