Micro Sem3 Mini2

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Author:
Anonymous
ID:
160447
Filename:
Micro Sem3 Mini2
Updated:
2012-06-28 22:47:32
Tags:
Micro
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Description:
HIV Parasite Micro sem3 mini2
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  1. HIV tropism
    • Macrophage tropic (CCR5)
    • T Lymphocyte tropic (CXCR4) 
  2. MO/EFFECTOR T-CELL TROPIC HIV
    • CCR5 receptor binding (R5)
    • Cell types with high CCR5 receptors:
    • macrophage and effector T cells  
  3. T-Resting/Effector T-cell tropic HIV
    • CXCR4 receptor binding (X4)
    • Cell types with high CXCR4 receptor:
    • Resting cell T-lymphocyte and Effector T-cell  
  4. attachment process
    what HIV and host proteins are involved? 
    • CD4-gp120
    • CXCR5/CCR5 helps to gp120 attachment
    • gp41 harpoons cell membrane 
  5. HIV genome activation in host cell signal
    • NF-kappaB acivation acivates viral transcription
    • same transcription factor activates the T-cell 
  6. HIV assembly
    see lidner slides
  7. HIV testing
    • ELISA for anti-HIV-ab
    • Western blot for same HIV for specific confirmation (anti-p24, gp41, gp120, gp160 HIV protein Ab)

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