Pipette holding predetermined amount of blood. Mix proper amounts of blood and diluent/lysing agent. Incubate mixture 10 min & invert to mix cells. Add to dropper assembly and add small amount to hemocytometer, making sure not to overflow moat around grid. Apply cover slip & observe on 40x mag.
How many squares make up the whole unopette grid? The corners? The middle?
9; 16; 400
What total mag is the WBC count done at? How do you get the total count of WBC?
View 9 primary cells w/ 10x objective, then add cells counted on both sides and divide by 2
When performing the RBC count, what feilds do you examine?
Center & four corner squares w/in the super square
What does the PCV measure?
% of whole blood composed of erythrocytes
What is the buffy coat composed of?
WBC & platelets
How is the HGB calculated?
Divide HCT by 3
What are the HCT and HGB normals for the canine and feline?
Canine: 37 - 55%; 12 - 18g/dl
Feline: 30 - 45%; 8 - 15 g/dl
What is methemoglobin/methemoblobinemia?
Methemoglobin is a form of hemoglobin containing oxidized iron & is inefficient at oxygen transport. Methemoglobinemia is an abnormal condition where an excess of methemoglobin is produced.
What causes methemoglobinemia?
Tylenol toxicity, hepatotoxicity, renal failure
What color is blood in animals with methemoglobinemia?
Chocolate brown; caused by methemoglobin spilling into the urine (methemoglobinuria)
What is MCV and what does it measure?
Mean corpuscular volume
Avg size of erythrocytes
What is MCH and what does it measure?
Mean corpuscular hemoglobin
Measurement of weight of hemoglobin in RBC and hemoglobins carrying capacity.
What are the 4 conditions possible for hemoglobin carrying capacity?