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What types of samples are used for clim chem?
- Whole blood
How soon should a sample be used after being collected and what should be done with the sample if it cannot be used w/in that time?
Used w/in the hour or frozen
How long should plasma and serum be centrifuged and at what rpm?
10 min @ 2000 - 3000 rpm
What specific component is serum lacking that plasma contains?
What can cause hemolysis?
- Moist syringe
- Vigorous mixing
- Forcing through needle
- Freezing whole blood
- Too much pressure on the syringe
What causes lipemia?
- Postprandial samples
What does lipemia interfer with?
What are 3 different analyzer types and which one is least affected by lipemia?
- Dry chemistry (least affected)
Who requires maintenance logs for QC?
OSHA & AAHA
When is a simple dilution performed?
When a serum chemistry is too high to read on the machine
What enzymes are released from damaged hepatocytes?
- Alanine aminotransferase (ALT)
- Aspartate aminotransferase (AST)
- Sorbitol dehydrogenase (SDH)
- Glutamate dehydrogenase (GLDH)
What enzymes are associated with cholestasis?
- Alkaline phosphatase (ALP)
- Gamma glutamyltranspeptidase (GGT)
What would you like to do?
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