Lecture 24.txt

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epaynich
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Lecture 24.txt
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2010-04-27 15:16:49
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Micro Lecture
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Micro 221 Lecture 24
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  1. Natural Immunity
    immune system response produced in body due to normal effects
  2. Artificial Immunity
    Response produced in response to unnatural events
  3. Active Immunity
    Producing your own Ig
  4. Passive Immunity
    recieving someone else's Ig
  5. Herd Immunity
    Protection the population has when most individuals are vaccinated
  6. Oral Vaccine
    Triggers B cell response in tissue to produce IgA
  7. Injected Vaccine
    triggers circulating B cells to result in IgG production
  8. Live attenuated Vaccine
    Weakened form of the infectious agent grows in the body but does not produce disease
  9. inactivated Vaccine
    Infectious agent is not present; mount weak immune response; only humoral
  10. Adjuvant
    enhances B7 production so that T cells become activated
  11. Toxoid Vaccine
    inactivated toxin used as a vaccine
  12. Acellular subunit vaccine
    vaccine that just injects a particular PMP to the body
  13. Polysaccharide capsule vaccine
    made with protein conjugate
  14. Edible Vaccine
    transgenic plants that you eat; like an oral vaccine
  15. DNA-Based vaccine
    DNA of pathogen are injected so you make the proteins and mount the immune response
  16. Antibody Titer
    concentration of antibodies in serum; titer of 1:256 is more concentrated that 1:16
  17. Preimmune serum
    serum before exposure to disease agent
  18. Convalescent Serum
    Serum as you're getting better from the infection while the antibody count is at the max
  19. Seroconversion
    4 fold change in titer between PIS and CS; evidence that you've been exposed
  20. Monoclonal Antibody
    Antibodies produced from a single B cell that recognize one particular epitope
  21. polyclonal antiserum
    produced from whole blood serum; contains IgG to many epitopes
  22. Serology
    study of blood serum
  23. Precipitin Test
    antibodies cross-link antigens forming large insoluble aggregates
  24. Immunodiffusion Test
    Antibody is added to center well of petri dish; antigens are in surrounding wells; they diffues and make a line of precipitin where they meet
  25. Immuneoelectrophoresis
    antigens are separated based on charge through electrophoresis, serum is added to trough, they diffuse and meet at a line of precipitin
  26. agglutination test
    large antigen-antibody cross linking; clumps antigen together
  27. ABO blood typing
    detected by clumpin in agglutination tests
  28. Lancefield typing
    enhanced visibility in agglutination by attaching latex beads to Fc part
  29. Direct Flourescent antibody test
    Bind known antibodies with flourescently tagged Fc ends to patient sample that may contain antigen
  30. Indirect Flourecent Antibody test
    1. bind antibodies from patient to known antigen 2. bind anti Fc secondary antibodies to primary antibodies
  31. direct elisa
    known antibodies are attached to the bottom of the well, patients serum is added, antibodies with enzymes are added, substrate for enzyme is added, if there is a color change the antigen is present in the serum. answers questions like does the patient have hepatitis
  32. Indirect elisa
    known antigen is attached to well, patient serum with possible antibodies is added, anti Fc antibodies with enzyme are added, substrate for enzyme is added, colored product= positive test, answers questions like has patient ever had hepatitis?
  33. Western blot
    separate antigens by electrophoresis, transfer to filter paper, add primary antibodies, add secondary antibodies, add substrate for enzyme, see where antigen was on gel
  34. primary antibody
    attaches to antigen; found in serum
  35. secondary antibody
    attaches to antibody that was in the patient serum, anti Fc, flourescently labeled or enzyme linked

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