lecture 4.txt

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epaynich
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16457
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lecture 4.txt
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2010-04-27 15:25:06
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Micro lecture
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Micro 221 lecture 4
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  1. lecture 4
    microscopy
  2. _ is how big something gets
    magnification
  3. _ is the minimum separation distance to see 2 objects clearly;d
    resolution
  4. _ is how distinct the specimen is from the background
    contrast
  5. _ is put below the slide and objective lens to change how light is refracted; all light on specimen is passed to lens
    immersion oil
  6. this microsope is when light passes through the condensor, is focused on the specimen, then passes to the objective and ocular lens
    bright field microscope
  7. this microscope has a condenser with metal plate blocking light directly on the speciman; light is reflected off the specimen
    dark field microscope
  8. this is part of a microscope has a metal plate that blocks light directly on a specimen
    dark field condenser
  9. this microscope retards and advances light waves to increase contrast of unstained bacteria
    phase contrast microscope
  10. this part of a microscope advances light by passing it through a bullseye like condenser
    phase plate
  11. this part of a microscope separates light into 2 perpendicularly polarized light
    wollastem prizm
  12. this microscope uses a wollasten prizm to separate light into polarized lens to detect 3d details of an organism
    DIC- differential interference contrast microscope
  13. this microscope uses fluorescent light to view a specimen
    fluorescence microscope
  14. this microscope uses confocal pinhole to view thick specimen
    confocal scanning laser microscope
  15. this is part of a microscope used in confocal scanning laser microscope to shut out light from all but one focal plane
    confocal pinhole
  16. this microscope is a light microscope that uses an electron beam instead of light beam; specimen must be extensively stained and fixed
    transmission electron microscope
  17. this reflects electrons off a specimen to show 3-d cutside of a specimen
    scanning electron microscope
  18. this microscope rotates around specimen using an electron source for images at various angles and are put together in 3d picture
    electron cryotomography
  19. this microscope has an electric field at tip of nanotube probe that monitors
    atomic force microscope
  20. this stain is the charge attraction that attaches color dye to organisms; basic dye attaches to � phospholipid heads
    simple stain
  21. this stain uses more than 1 stain to differentiate between cell properties
    differential stain
  22. this stain differentiates color that attaches based on thickness of peptidoglycan
    Gram stain
  23. this stain differentiates based on waxy coating of bacteria
    acid fast stain
  24. this stain stains the background of things that can�t be stained
    negative stain

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