lecture 9.txt

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epaynich
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16462
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lecture 9.txt
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2010-04-27 15:27:33
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Micro lecture
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Micro 221 lecture 9
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  1. lecture 9
    enumeration of growth
  2. period of time with little growth where bacterium adopt to new conditions
    lag phase
  3. period of extreme growth and lots of binary fission
    exponential phase
  4. time when nutrients run out, toxin are produced, or crowding occurs when bacteria feed off dying bacteria
    stationary phase
  5. period where the overall number of bacteria decrease
    death phase
  6. number of bacteria growing per hour
    growth rate constant
  7. what is the formula for generation time and what does each mean respectively N=Ne^(ut)
    final cells = one cell * growth rate * time
  8. growth factors bacterium produce during exponential phase such as acid, gas and ethanol
    primary metaboblite
  9. growth factors bacterium produce during stationary phase to compete with other bacteria such as antibiotics and bacterioins
    secondary metabolite
  10. cells well adapted to get nutrients from other cells during the death phase
    persister cells
  11. culture in a flask which gives all nutrients in the beginning and waste builds up
    batch culture
  12. using a chemostat nutrients and waste controlled to determine growth rate
    continuous culture
  13. apparatus used for a continuous culture
    chemostat
  14. counts all cells alive and dead
    direct cell count
  15. grid which allows for counts of immotile cultures; not useful for large numbers
    petroff hauser chamber
  16. electrode measures interruption of electric current as cells pass through a counter; not good for soil samples
    coulter counter
  17. count only live cells in colonies/mL
    viable cell count
  18. spread diluted sample over agar for a viable cell count of colonies
    spread plate
  19. pour diluted culture within agar for viable cell count
    pour plate
  20. large volumes can be counted on filter paper
    membrane filter
  21. fifteen test tubes with different dilutions are used to determin probability of growth
    most probably number
  22. how cloudy a solution of bacteria is based on measurements of spectrometer; limited to dense bacterium
    turbidity
  23. used to measure acid from fermentation of bacterium
    biochip
  24. acid from fermentation conducts elections which results in __
    conductivity
  25. upside down test tube used to collect gas bubbles to measure bacterium
    durham tube
  26. enzyme used to nonquantatively tell if live cells remain in a culture by reacting with ATP to produce light
    luciferase

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