The flashcards below were created by user
minna528
on FreezingBlue Flashcards.
-
Why is it important to record the results from the o-tolidine test within 10-15 seconds
To redue the chance of recording a false positive: Natural oxidation from the air can cause the colored form to appear after longer time intervals even in the absence of blood
-
What is known to cause a premature positive result with tetramethylbenzidine
Bleach
-
T/F unlike LMG, TMB is a conclusive test
False
-
The color change for catalytic color test for blood should occur
After the addition of the oxidizer
-
T/F Increasing the number of steps in a catalytic color test can increase specificity
True
-
Is the Kasle-Meyer test qualitiative, quantitative or both?
It is only qualitative
-
When can a catalytic color test yeild a positive result for hemoglobin with saliva?
When there are trace amounts of blood in the saliva
-
Horseradish will give a false positive for blood after?
The addition of the oxidizer
-
Most of the forensic literature points to________ as the most specific presumptive test for blood
Leucomalachite green
-
Animal blood with give a ____ result with O-tolidine
positive
not species specific
-
Microcrystalline test for blood confirm the presence of:
Heme derivatives
-
Hemochromagencrystals are________ in appearance.
bright red and feathery
-
A reason for a negative Teichman test of a known blood stain may be
the sample may have been over heated
-
Which microcrystalline test does not require hight temperatures for crystallization to occur?
Takayama
-
Hematin crystal formation in a bloodstain can be induced by using salt____ and ____ under _____conditions
Salt
Heat
Acidic
-
The specificity of antigens and antibodies is based on their:
Structure
-
Where on the RBC are antigens located?
on the membrane of the red blood cell
-
A person's ABO blood type refers to the ____ present
antigen
-
Type O blood contains what antibodies?
Anti-A and Anti-b
-
Which presumptive test for blood is considered not to be carcinogenic?
Phenolphthalein
-
Kastle Meyer test is also known as?
Phenolphthalein test
-
McPhail's reagent is also known as
Leucomalachite green
-
A leuco dye in the _____ form is colorless
reduced
-
A 1:2 serial dilution is started by using 1mL of blood and adding ____ of water
1mL
-
For blood samples used for species testing the blood should be diluted with buffer until a _____ color is observed
Straw
A sample too strong may overwhelm the antibody
-
Cross reactivity usually occurs with:
Closely related species
-
In the Oucherlony diffusion method, more than one band may form if:
different components of the extract have different diffusion coefficients
-
In a positive chromatographic immunoassay, the Test area "captures" _______ that formed in the sample well and causes a visible reaction.
mobile antigen-antibody complexes
-
A false negative result in a chromatographic immunoassay due to large amounts of _____ is called the _____ effect.
antigen; high dose hook
-
Acid phosphatatse catalyzes the breakdown of _____ which then reacts with a _____ to produce a colored compound
alpha-naphthyl phosphate ; colorless azo dye
-
A stain is considered to be negative for AP if no color change associated with the Brentamine reaction occurs after:
5 minutes
-
A weak or slow reaction using the AP Spot Test means that the stain:
Could be vaginal fluid
-
The presence of _____ can inhibit the fluorescing of semen stains while using ALS
bloodstains
-
What is the best order to process a possible semen stain?
- Visual exam
- AP testing
- PSA testing
-
What color do nucli stain with H&E?
Purple
-
Rapid Stain IDentification immunochromatographic test kits detect _____________.
SVSA
-
The ABAcard immunochromatographic test kits for p30 are reported to be sensitive up to approximately:
1:1 million dilution
-
What color do sperm heads stain with Christmas tree?
Red
-
What are common things mistaken for sperm heads?
bacteria, e cell nucli and yeast
-
Amylase is an enzyme that digests:
Starch
-
How do you differentiate between AMY-1 and AMY-2?
gel electrophoresis
-
The RSID immunochromatographic test for saliva is sensitive to:
0.001mL of saliva
-
T/F The starch-iodine test for amylase is semi-quant
True!
-
Which is more specific SALIgAE or Phadebas tube test?
SALIgAE
-
Which test for saliva has an unknown mechanism?
SALIgAE
-
Urine is yellow due to:
urochrome
-
Normal healthy males urine is a poor source of:
cellular materials
-
Iodine solution or PAS stain can be used to detect:
the presence of glycogentated e-cells
-
Glycogentated e-cells stain:
brown
-
Normal adults produce_____ of urine a day
.75-2 liters
-
If a slide constains more that _____% glycogenated e-cells it is most likely a vaginal sample
30%
-
What does DMAC react with?
Urea
-
Swabs collected from a victim of a vaginal sexual assault approximately 48 hours after the assault will _____ contain semen evidence
possibly
-
The typical maximum time frame in which sperm can be observed in a living victim after an oral assault is _________.
5 to 6 hours
-
Teichman reagent consists of:
glacial acetic acid and a halide
-
The teichmann reagent converts_______ to ______
Hemoglobin to hemin (hematin)
-
The Takayama test uses _____ and ____
pyridine and sugar
-
The Takayama test induces the formation of ______
Hemochromogen
-
Hemochromogen is fromed when ______ from hemoglobin reacts with pyridine
ferrous iron
-
What are the reagents used for the presumptive testing of blood?
- Phenolphpthalein
- Tetramethylbenzidine
- Leucomalachite green
- orthotolidine
-
The Ouchterlony method relies on the radial double diffusion of:
Ab and Ag
-
Immunoprecipitate are visible where:
Ab-Ag complexes are formed
-
Azo dyes are:
- chemcial compounds with a nitrogen functional group
- usually stable
- have vivid colors
-
Reporting a positive result for saliva (general)
Analysis of ___ gave chemical indications for the presence of amylase, a component of saliva
-
Negative Saliva result
No amylase activity was observed/ Amylase, a constituent of saliva, was not detected on____
-
-Amylase diffusion quant. positive results for amylase equal/larger to 1:100 dilution
-between 1:100-1:500 dilution
-less than 1:500
Elevated levels of amylase were detected on____, which indicates presence of saliva
Amylase was dectected on ____, Amylase at this level has been observed in saliva as well as other biological fluids
Low levels of amylase were detected on____, Amylase activity at this level is inconclusive for the presence of saliva
-
Positive AP
Analysis or ___gave presumptive chemical indications for the presence of acid phosphatase, a component of semen
-
Positive Microscopic ID of semen
Microscopic examination of ____demonstrated the presence of spermatozoa.
-
Postitive AP and Negative Microscopic and Positive P30
Semen was identified on_____, however micrscopic examination failed to demonstrate the presence of spermatozoa
-
Positive AP Negative microscope and negative P30
No semen was identified on______
-
Negative AP
No semen was identified on________
-
Postitive catalytic color test for blood
Analysis of _____gave presumptive chemical indications for the presence of blood
-
Postive catalytic color test and positive Takayama/Teichman
Blood was identified on______
-
Positive catalytic color test for blood and Neg Taka/Tiech
Preliminary tests indicated blood on_____, but further tests could not confirm the identiy of blood
-
Negative catalytic color test for blood
Analysis of ____failed to give preumptive chemical indications for the presence of blood
-
Speicies testing postive results (human antiserum)
Analysis of ____indicates the presence of human proteins
-
Speices testing (immunoassay such as hemoglobin or human glycophorin A)
- Blood was identified on ___, and:
- human glycophorin A activity was detected.
- human hemoglobin activity was detected, Blood from upper primates and domestic ferrets may also cause postive reults with this assay.
-
Chromotographic Immunoassay
A dye labled______ ______ hemoglobin_____ present in test strip
monoclonal antihuman hemoglobin antibody is present in the test strip
-
Chromatographic Immunoassay
Test area has ______ ______ ______ hemoglobin that captures the Ag-Ab Complex and Ab-Ag-Ab sandwich is formed
The test area has immobilized polyclonal antihuman hemoglobin that captures the Ag-Ab complext and forms a Ab-Ag-Ab sandwich
|
|