Enzymes.txt

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• author "Joshua Ellis"
• tags "HUCM"
• description ""
• fileName "Enzymes"
• freezingBlueDBID -1.0
• What is the Michaelis -Menten Equation

Vo= Vmax[S]/(Km+[S]
1. Vo= initial velocity
• Vmax= maximal velocity
• Km= Michaelis constant = (k-1 + k2)/k1
• [S]=substrate concentration
2. What is Km? Relate it to Vmax.
• Michaelis constant
• (k-1 +k2)/k1 (all the rate constants below)

• k1 k2
• E+S <---> ES ---> E+P
• k-1
• Does not change with concentration of enzyme. Basically equal to reaction velocity = 1/2 Vmax
3. What does a low and high Michaelis constant mean?
• Small Km = high affinity of the enzyme for substrate. (low concentration of substrate need to half saturate the enzyme--reach v=1/2vmax)
• Large Km = low affinity. Cuz a high concentration is required.
4. What is the relationship to enzyme concentration and velocity of reaction?
ALWAYS directly proportional. If you half [E] then the Vo and Vmax will also be halved
5. How can you determine the order of the reaction?
• When [S] is much less than Km velocity is proportional to [S]-----FIRST ORDER
• When [S] is much greater than Km velocity is constant and equal to Vmax (independent of [S])----- ZERO ORDER
6. How do you make the Lineweaver-Burke Plot? What does it tell you? What is the equation?
Its the reciprocal of the Michaelis Menten equation

It can tell you the Km and Vmax (as x and y intercepts)

1/vo= Km/(Vmax[S]) + 1/Vmax
7. Reversible inhibitors have what type of bond?
Non-Covalent
8. What is competitive inhibition? What is the effects on Vmax, Km, and Lineweaver Burke Plot
Binds to active site

• Vmax is the same
• Increased Km because need more [S]
• Graph y intersect is same but x intercept is increased (obvious slope change)
9. What is non-competitive inhibition? What is the effects on Vmax, Km, and Lineweaver Burke Plot
non active site binding

• Vmax decreased
• Km is the same

The graph x intercept is the same
 Author: Anonymous ID: 164974 Card Set: Enzymes.txt Updated: 2012-08-04 18:48:45 Tags: HUCM Folders: Description: enzymes from book Show Answers: