Card Set Information
Sexual Assault lecture
Actual sperm released from a substrate may be as low as ______ of sperm deposited in stain
Certain substrates, such as cotton, hold on to sperm better than others
What are the 2 techniques for generateing a DNA profile from stained slides?
1. xylene bath to remove coverslip/mounting mediua, tranfer bio material to extraction tube by scraping/swabbing
2. laser capture microdissection
Who collects sexual assault kit samples?
SANE nurses (Sexual Assualt Nurse Examiner)
Most major hospitals
How long do Sexual Assault kits take to collect?
How long does it take to analyize a kit?
several hours to several months
How long are the kits retained?
Until the case has been adjudicated
until statue of limitaions has expired
indefinietly in homicide cases
maybe discarded sooner if victims requests or if case isnt reported to police
What are the steps?
Known blood sample
Foreign material collections
head/pubic hair combings
head/pubic hair standards
genital,perianal, vaginal and anal swabs and smears
Why is time since intercourse important?
-When IDing the presence of semen isnt enough
-a consensual partern may be a factor
-sperm cells can be unrealted to assualt
-aides in establishing time/circumstances of death
Typical post-coital interval (PCI)
intact sperm in vagina
PCI sperm heads
PCI sperm heads in cervix
PCI P30 in vagina
PCI AP in vagina
Studies show that ____% of rapists do not ejaculate after _____ minuites of vaginal penetration
The vagina is a ___ enviornment
the cervix is a ______ environment
T/F Semen identified on anorectal swabs indiacts anal penetration
–Could be a result of contamination from vaginal
–Could be due to collection technique when
inserting swabs into the rectum
What factors effect PCI?
Stage in menstrual cycle
washings, swabs, smears, liquid saliva
temp and humidity