Intro to Histo.

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  1. Eosin vs. Hematoxylin?
    Eosin for basic or acidophilic substances (proteins)

    Hematoxylin is for acidic or basophilic substances (DNA)
  2. What is Periodic acid-Schiff used for?
    PAS (or HIO4) turns carbohydrates into aldehydes which can be tagged purple schiff reagent
  3. What is the purpose of enzyme histochemistry?
    Soluble, non-colored substrate are converted to non-soluble, colored products via enzyme activity.

    Thus, the quantity of precipitate is proportional to enzyme activity
Author:
sbandzar
ID:
170244
Card Set:
Intro to Histo.
Updated:
2012-09-11 16:58:27
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Sickles
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med school
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