Streak Plate Method of Isolation
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Why do we perform isolation streaking?
To produce individual colonies - then transfer to sterile medium to start a pure culture.
Describe technique used for isolation streaking.
- # of streaks: Quad streak - 4
- where on argar plate: 90 degrees 3 times and then up the middle
- tool used: inoculating loop
What is CFU & why do we use this term in lab?
CFU: term used to define cells that produce a colony when transferred to plated media.
What would be a situation we wouldn't need to use isolation streaking?
If it was already a pure culture.
What would you like to do?
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