Fluid Thioglycolate Medium
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What is FTM made of & why is it used?
FTM: yeast extract, pancreatic digest of casein, dextrose, sodium chloride, sodium thioglycollate, L-cystine, agar, resazurin, water.
Used to determine microbial growth. To test the aerotolerance of bacteria.
cultivation of strict anaerobes & microaerophiles.
How is media inoculated?
Take bacteria on loop and go down to the bottom in a swirling motion.
What are the names given to different types of bacteria based on their oxygen requirements?
- OBLIGATE AEROBE: require oxygen - grow at top.
- OBLIGATE ANAEROBE: no oxygen - grow at bottom.
- MICROPHILE: tolerates oxygen in small amt. - grow upper middle.
- FACULATIVE ANAEROBE: doesn't need oxygen but can use it if needed - grows bottom to middle.
- AEROTOLERANT ANAEROBE: not harmed by oxygen - grows throughout medium.
Which genera of bacteria grow in anaerobic environment?
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