Ch 3 Micro

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XQWCat
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173834
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Ch 3 Micro
Updated:
2012-09-27 09:07:44
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Microbiology
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Microbiology Chapter 3 test questions
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  1. Which of the following is NOT equal to 1 m?
    A) 106 μmB) 109 nmC) 10 dmD) 100 mmE) 0.001 km
    D
  2. What structure does light pass through after leaving the condenser in a compound light microscope?A) Ocular lensB) Objective lensC) SpecimenD) Illuminator
    C
  3. Which of the following pairs is mismatched?A) Gram-negative bacteria – negative stainB) Iodine – mordantC) Alcohol-acetone – decolorizerD) Acid-alcohol – decolorizerE) Crystal violet – simple stain
    A
  4.  Place the steps of the Gram stain in the correct order: 1-Alcohol-acetone; 2-Crystal violet; 3-Safranin; 4-Iodine.A) 1-2-3-4B) 2-1-4-3C) 2-4-1-3D) 4-3-2-1E) 1-3-2-4
    C
  5. Which of the following pairs is mismatched?A) Alcohol-acetone – decolorizerB) Crystal violet – basic dyeC) Safranin – acid dyeD) Iodine – mordantE) Carbolfuchsin – basic dye
    C
  6. The counterstain in the acid-fast stain is
    A) A basic dye.B) An acid dye.C) A negative stain.D) A mordant.E) Necessary to determine acid-fast cells
    A
  7. The purpose of a mordant in the Gram stain isA) To remove the simple stain.B) To make the bacterial cells larger.C) To make the flagella visible.D) To prevent the crystal violet from leaving the cells.E) To make gram-negative cells visible
    D
  8. Place the following steps in the correct sequence: 1-Staining; 2-Making a smear; 3-Fixing.A) 1-2-3B) 3-2-1C) 2-3-1D) 1-3-2E) The order doesn't matter
    C
  9. The best use of a negative stain isA) To determine cell size.B) To determine cell shape.C) To determine Gram reaction.D) To see endospores.E) A and B
    E
  10. Simple staining is often necessary to improve contrast in this microscope.A) Compound light microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) Fluorescence microscopeE) Electron microscope
    A
  11. Which microscope is used to see internal structures of cells in a natural state?A) Compound light microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) Fluorescence microscopeE) Electron microscope
    B
  12. Which of the following microscopes uses visible light?
    A) Confocal microscope
    B) DIC
    C) Fluorescence microscope
    D) Scanning acoustic microscope
    E) Scanning electron microscope
    B
  13. Which microscope achieves the highest magnification and greatest resolution?A) Compound light microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) Fluorescence microscopeE) Electron microscope
    E
  14. In this microscope, the observer does NOT look at an image through a lens.A) Compound light microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) Fluorescence microscopeE) Electron microscope
    E
  15. This microscope produces an image of a light cell against a dark background; internal structures are NOT visible.A) Compound light microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) Fluorescence microscopeE) Electron microscope
    C
  16. Which of the following is NOT correct?A) 1 μm = 10-6 mB) 1 nm = 10-9 mC) 1 μm = 103 nmD) 1 μm = 10-3 mmE) 1 nm = 10-6 μm
    E
  17. The counterstain in the Gram stain isA) A negative stain.B) A mordant.C) A basic dye.D) An acid dye.E) Necessary to determine the Gram reaction.
    C
  18. Which microscope can be used to visualize DNA or botulinum toxin?A) Compound light microscopeB) Phase-contrast microscopeC) Scanning tunneling microscopeD) Confocal microscopeE) Scanning electron microscope
    C
  19. Which microscope is used to observe a specimen that emits light when illuminated with an ultraviolet light?A) Compound light microscopeB) Phase-contrast microscopeC) Darkfield microscope
    D
  20. Which microscope is most useful for visualizing a biofilm?A) Compound light microscopeB) Phase-contrast microscopeC) Atomic force microscopeD) Scanning acoustic microscopeE) Transmission electron microscope
    D
  21. Which microscope takes advantage of differences in the refractive indexes of cell structures?A) Compound light microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) Fluorescence microscopeE) Electron microscope
    B
  22. The appearance of gram-positive bacteria after addition of the first dye in the Gram stain.A) PurpleB) RedC) ColorlessD) Brown
    A
  23. The appearance of gram-negative bacteria after addition of the mordant in the Gram stain.A) PurpleB) RedC) ColorlessD) Brown
    A
  24. The appearance of gram-negative bacteria after addition of the decolorizing agent in the Gram stain.A) PurpleB) RedC) ColorlessD) Brown
    C
  25. The appearance of gram-positive bacteria after adding the counterstain in the Gram stain.A) PurpleB) RedC) ColorlessD) Brown
    A
  26. The appearance of gram-negative bacteria after completing the Gram stain.A) PurpleB) RedC) ColorlessD) Brown
    B
  27. Which of the following pairs is mismatched?A) Confocal microscope – produces a three-dimensional imageB) Darkfield microscope – uses visible lightC) Fluorescence microscope – uses a fluorescent lightD) Scanning electron microscope – produces a three-dimensional imageE) Scanning tunneling microscope – allows visualization of atoms
    C
  28. What is the total magnification of a chloroplast viewed with a 10x ocular lens and a 45x objective lens?A) 4.5xB) 10xC) 45xD) 100xE) 450x
    E
  29. You suspect a 100-nm structure is present in a cell. Which of the following provides the lowest magnification that you can use to see this structure?A) Brightfield microscopeB) Darkfield microscopeC) Transmission electron microscopeD) Phase-contrast microscopeE) Scanning electron microscope
    E
  30. Which microscope uses two beams of light to produce a three-dimensional, color image?A) Fluorescence microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) DIC microscopeE) Electron microscope
    D
  31. Which microscope is used to see intracellular detail in a living cell?A) Fluorescence microscopeB) Two-photon microscopeC) Atomic force microscopeD) Transmission electron microscopeE) Brightfield microscope
    B
  32. In which microscope does the image look like a negative stain?A) Fluorescence microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) Two-photon microscopeE) Scanning acoustic microscope
    C
  33. Which microscope is used to see detail of a 300-nm virus?A) Fluorescence microscopeB) Phase-contrast microscopeC) Darkfield microscopeD) DIC microscopeE) Electron microscope
    E
  34. Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin. Through the microscope, the green structures areA) Cell walls.B) Capsules.C) Endospores.D) Flagella.E) Can't tell.
    C
  35. Cells are differentiated after which step in the Gram stain?A) SafraninB) Alcohol-acetoneC) IodineD) Crystal violet
    B
  36. You find colorless areas in cells in a Gram-stained smear. What should you do next?A) An acid-fast stainB) A flagella stainC) A capsule stainD) An endospore stainE) A simple stain
    D
  37. What Gram reaction do you expect from acid-fast bacteria?A) Gram-positiveB) Gram-negativeC) Both gram-positive and gram-negativeD) Can't tell
    A
  38. Bacterial smears are fixed before staining toA) Kill the bacteria.B) Affix the cells to the slide.C) Make their walls permeable.D) A and B.E) Accept stain.
    D
  39. The resolution of a microscope can be improved by changing theA) Condenser.B) Fine adjustment.C) Wavelength of light.D) Diaphragm.E) Coarse adjustment.
    C
  40. Van Leeuwenhoek's microscope magnified up to 300x. This was a(n)A) Electron microscope.B) Phase-contrast microscope.C) Simple microscope.D) Confocal microscope.E) Compound microscope.
    C
  41. The purpose of the ocular lens is toA) Improve resolution.B) Magnify the image from the objective lens.C) Decrease the refractive index.D) Increase the light.E) Decrease the light.
    B
  42. The signal molecule produced in quorum sensing isA) A counterstain.B) An inducer.C) Light.D) A simple stain.E) An endospore.
    B

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