Explain how fusion of the vesicle and target membranes is accomplished using SNAREs.
One v-SNARE interacts with with three t-SNAREs to form a trans-SNARE complex (two sets of complexes per vesicle). They interact to form a coiled-coil structure which brings the vesicle very close to target membrane. After fusion, the trans-SNARE complexes are now cis-SNARE complexes because all the proteins are on the same membrane. The cis-SNARE complexes must be unwound to recycle the proteins- requires GTP.