- Nucleotides serve as carriers of activated intermediates in the synthesis of
Carbohydrataes - e.g. UDP-glucose
Lipids - e.g. CDP-choline
Conjugated proteins – e.g. Helicase enzymes
- Nucleotides also act as “second messengers”
e.g. cyclic AMP
- Nucleotides hold onto and transmit directed energy
(i.e. ATP and GTP as “Energy Currency”)
T and U differ only in that Thimine................
Thimine has an additional (1 carbon) methyl group
How purine is degraded?
Phosphodiesterase enzymes cleave oligosaccharides into mononucleotides
Nucleotidase enzymes then remove the phosphate, giving nucleosides
- ............. is the “end product” of purine breakdown
- Formed primarily in...................
Uric acid is the “end product” of purine breakdown
Formed primarily in intestinal mucosal cells
- Uric acid is formed from..................
Hypoxanthine
What happens when Uric Acid crystallizes?
- It deposits and forms Gout- If it deposits in the Kidney form Kidney Stones
The vast majority of gout happens because of inadequate excretion
1- Defects in kidney function
2- Lactate acidosis (which could lower the pH of urine and cause more urate to become insoluble)
3- Environmental factors, or taking certain drugs
How you treat Gout?
1- Lessen the amount of inflammation (i.e use steroids, NSAIDS)
2- Get rid of the excess uric acid
a- Use Uricosuric Agents to increase renal excretion. -------Probenecid
-------Sulfinpyrazone
B- Use Allopuranol (a structural analog of hypoxanthine) to inhibit synthesis of Uric Acid
3- Make it more soluble
----Urate Oxidase – that converts Uric Acid into Allantoin
---Allantoin is five to ten times more soluble than uric acid, so renal excretion is more effective
The key regulated step in the pyrimidine synthesis is making of ----------------
Carbamoyl phosphate
There are 2 Carbamoyl Phosphate Synthesis
(CPS) enzymes (I and II).
CPS I contributes to synthesis.............
CPS II contributes to synthesis..............