Card Set Information
MLT automation hematology
automation in hematology for MLT
Coulter employs what type of methodology?
The height of each pulse is proportional to what? What is this called?
The volume/size of each cell in fL
This preserves cell shape in the coulter counter.
Isotonic conductive solution
The number of pulses corresponds to what?
Number of cells
This produces a coincidence error that is automatically corrected.
Two or more cells passing thru the aperture simultaneously
This excludes pulses below or above the adjustable height of the counters.
Threshold setting or pulse discriminator
What are the two counting chambers and what do they count?
RBC bath (RBCs and Plt)
WBC bath (WBC and Hgb)
This is measured colorimetrically at 540nm.
How many apertures are used for counting?
If all three results disagree, none are accepted what is this called?
Vote out (---)
What are the components of a three part differential?
Which differential uses VCS technology?
Five part WBC differential
What does VCS stand for?
This part of VCS is based on electronic impedance and provides information on size of cells.
This part of VCS uses high-frequency electromagnetic probe that provides information on internal constituents.
This part of VCS helps differentiate between cells of like sizes.
This part of VCS uses monochromatic light scatter at a forward angle.
This part of VCS helps determine cell surface characteristics like morphology and granulation.
What are the components of a five part differential?
How are abnormalities identified?
What are the three types of system messages in the flagging system?
These flags are generated by the instrument and identify abnormal cell population or distributions.
Dimorphic RBCs, immature cells, and blasts are examples of what type of message?
These messages are set by the individual laboratory and define the limit of normal values.
Leukocytosis, thrombocytopenia, or small/large platelets are examples of what message?
This message indicates whether the WBC, RBC and platelet populations are normal or abnormal.
This code indicates that values exceed printable ranges.
This code indicates that a total vote out has occurred.
What should be done if a vote out occurs?
Check for clots
This code indicates incomplete computation, the sample should be rerun.
If a WBC value is highlighted a smear should be made and checked for what three things?
NRBC, platelet clumping and unlysed RBC’s can cause falsely ______ WBC.
Clotting, smudge cells, and damaged WBC can cause falsely _____ WBC.
Giant platelets and a WBC count over 50 can cause falsely _____ RBC.
Clotting, in vitro hemolysis, and microcytosis can cause a falsely _______ RBC.
In vitro hemolysis, WBC counts over 50, hyperbilirubinemia and lipemia can cause falsely ______ hgb.
Clotting can cause a falsely ______ hgb.
This is a diagram or graphic representation of frequency distribution.
Histograms are based on ___________.
On a histogram lymphocytes are in what cell population.
On a histogram mononuclears are in what cell population.
On a histogram granulocytes are in what cell population.
Particles with cell volume >36 but =/< 360fl are identified as what?
A tail on an RBC histogram may indicate what three conditions?
Doublets and triplets
Particles between 2-20fl are counted as _____.
To have a valid platelet count, what must be obtained?
In no fitted curve is obtained for platelet how is the count obtained?
This visual aid helps identify possible immature cells.
Which slide stainer can be operated manually?
How many slides is the hemastainer capable of holding?
What are the six stations for the hemastainer and how long is each station?
Methanol- 15 sec
Wright stain- 2 min
Wright stain/ phosphate buffer- 5 min
DI water- 20 sec
Phosphate buffer- 1 min
How long does the entire hemastainer process take including drying?
In which stainer are slides carried through the staining procedure face down on a platform?
Hema-tek 100 stainer
What is the rate of staining in a hema-tek 100?
One slide per minute
What are the four stations on the hema-tek 100?
How often should phosphate buffer and solutions be replaced in a stainer?