blood bank blood storage

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Author:
egoranson
ID:
17771
Filename:
blood bank blood storage
Updated:
2010-06-04 23:58:12
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blood bank blood storage
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blood bank blood storage
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  1. Red blood cells/whole blood storage requirements?
    • 21 days - CPD
    • 35 days - CPDA-1
    • 42 days - Additive soln
    • All at 1 - 6 deg C
  2. Frozen RBCs storage requirements?
    10 years at -65 deg C, 24 hours post thaw
  3. Washed RBCs storage requirements?
    24 hours at 1-6 deg C
  4. Platelets storage requirements?
    5 days at 20-24 deg C with gentle aggitation
  5. WBCs storage requirements?
    24 hours at 20-24 deg C, without aggitation
  6. FFP storage requirements?
    • 1 year at -18 deg C
    • 7 years at -65 deg C
    • 24 hours at 1-6 deg C after thaw
    • Can be used as thawed plasma following that, low in factors 5 and 8
  7. Cryoprecipitate storage requirements?
    • 1 year at -18 deg C
    • 4 hours (after pooling) at 20-24 deg C
  8. RBC QC
    Hct < 80% (all)
  9. RBC leukoreduced QC
    < or = 5 x 10^6 WBC in 95% and retain 85% RBCs
  10. Platelets (PC) QC
    > or = 5.5 x 10^10 and pH > or = 6.2 in 95%
  11. Apheresis platelets QC
    > or = 3 x 10^11 and pH > or = 6.2 in 90%
  12. Apheresis platelets leukoreduced QC
    <5.0 x 10^6 WBC in 95%
  13. Cryoprecipitate QC
    • > or = 80 IU of Factor 8
    • > or = 150mg of Fibrinogen
  14. Granulocyte concentrate QC
    > or = 1 x 10^10 in 75%
  15. Minimum irradiation dose
    2500 cGys of gamma radiation
  16. Storage requirements of RBCs following irradiation
    28 days post irradiation or expiration date, whatever comes first
  17. What platelets to use for NATP?
    Washed maternal platelets due to maternal IgG against HPA-1A antigen (anti-PLA1) on fetal plts
  18. Risk factors for TA-GVHD
    • Immunosuppression (Tcell deficiencies, Bone marrow tx, Fludaribine)
    • Intrauterine and premature infants
    • Hodgkins lymphoma + other heme malig
    • HLA matched product or 1st degree relative
  19. Why used leukoreduced products?
    • Prevent HLA immunization
    • Prevent CMV transmission
    • Prevent febrile non-hemolytic reactions

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