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The flashcards below were created by user
egoranson
on FreezingBlue Flashcards.
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Red blood cells/whole blood storage requirements?
- 21 days - CPD
- 35 days - CPDA-1
- 42 days - Additive soln
- All at 1 - 6 deg C
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Frozen RBCs storage requirements?
10 years at -65 deg C, 24 hours post thaw
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Washed RBCs storage requirements?
24 hours at 1-6 deg C
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Platelets storage requirements?
5 days at 20-24 deg C with gentle aggitation
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WBCs storage requirements?
24 hours at 20-24 deg C, without aggitation
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FFP storage requirements?
- 1 year at -18 deg C
- 7 years at -65 deg C
- 24 hours at 1-6 deg C after thaw
- Can be used as thawed plasma following that, low in factors 5 and 8
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Cryoprecipitate storage requirements?
- 1 year at -18 deg C
- 4 hours (after pooling) at 20-24 deg C
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RBC leukoreduced QC
< or = 5 x 10^6 WBC in 95% and retain 85% RBCs
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Platelets (PC) QC
> or = 5.5 x 10^10 and pH > or = 6.2 in 95%
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Apheresis platelets QC
> or = 3 x 10^11 and pH > or = 6.2 in 90%
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Apheresis platelets leukoreduced QC
<5.0 x 10^6 WBC in 95%
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Cryoprecipitate QC
- > or = 80 IU of Factor 8
- > or = 150mg of Fibrinogen
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Granulocyte concentrate QC
> or = 1 x 10^10 in 75%
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Minimum irradiation dose
2500 cGys of gamma radiation
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Storage requirements of RBCs following irradiation
28 days post irradiation or expiration date, whatever comes first
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What platelets to use for NATP?
Washed maternal platelets due to maternal IgG against HPA-1A antigen (anti-PLA1) on fetal plts
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Risk factors for TA-GVHD
- Immunosuppression (Tcell deficiencies, Bone marrow tx, Fludaribine)
- Intrauterine and premature infants
- Hodgkins lymphoma + other heme malig
- HLA matched product or 1st degree relative
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Why used leukoreduced products?
- Prevent HLA immunization
- Prevent CMV transmission
- Prevent febrile non-hemolytic reactions
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