Nitrate Reduction TestStab buttStreak slant(we have agar, not broth)•
Testing for:•A. Reduction of nitrate, NO3 to nitrite, NO2 (Which is detected by reagents)•Phase 2:•A. Add 5 drops sulfanilic acid, then 5 drops a –naphthylamine.•Phase 2 results:•A. Red color indicates nitrite, NO2 is present –Positive Test ( NO3 was reduced ).•B. Other compounds ( not detected by reagents)•Phase 3:•B. Add a “Pinch” of powdered zinc.•Phase 3 results:•B. If NO3, was not used, it will be reduced to NO2 by zinc: -Red / Pink color is a negative test.•-Clear reagents indicate a positive test.