Diffusion & Emzyme Lab

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Diffusion & Emzyme Lab
2012-10-23 06:43:29
BI 253 Lab

BI 253 Diffusion & Enzyme Lab
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  1. A plasma membrane allowing water to pass freely through but regulating mvmt of solvents is called ___ ___.
    Selective Permeability
  2. Water is a universal dissolving agent making it the ____ in a solution.
  3. The substances that are dissolved in a solution are the ____.
  4. What is the physical process where molecules move fr an area of high concentration to an area of low?
  5. What is osmosis?
    Diffusion of water thru selectively permeable membrane fr area of high concentration to an area of lower concentration.
  6. Solutes that cannot cross a membrane are called ___ ___.
    Nonpenetrating Solutes
  7. A hypertonic solution is one in which . . .
    Solute concentration is higher outside the cell than inside giving it a greater osmolarity
  8. A hypertonic solution causes water to . . .
    Flow out of the cell & eventually crenate
  9. A solution w/greater concentration of solute inside the cell (lower osmolarity) is called ____.
  10. A hypotonic solution cause water to . . .
    Flow into the cell and eventually lysis
  11. What is the term for solute concentration expressed in molarity?
  12. 2 solutions that are said to be in equilibrium are called ____.
  13. What form of energy drives diffusion?
    Intrinsic molecular kinetic energy
  14. When a protoplast, in a plant cell, shrinks & pulls away fr the cell wall due to being placed in a hypertonic solution ____ occurs.
  15. A plant cell exclusive of the cell wall is called a ____.
  16. When a plant cell is placed in hypotonic solution, water flows in expanding the protoplast against the cell wall causing what?
    Turgor Pressure
  17. What is water potential in plant cells?
    Combined force created by solute concentration & physical pressure
  18. A plant cell that has undergone plasmolysis is said to be ____.
  19. Lg particles suspended in water in motion is traditionally called ___ ___.
    Brownian Mvmt
  20. What are most enzymes made up of?
  21. pH is a measure of what?
    H⁺ in solution
  22. How does salt affect an enzyme?
    Like pH there is an optimal salt level
  23. The enzyme catecholase catalyzes a reaction where ___, the substrate becomes ___ (product).
    • Catechol
    • Benzoquinone
  24. What does benzoquinone look like?
    Reddish-brown product
  25. What instrument can we use to measure how much color change happens during a reaction?
  26. How does a Spectronic 20 measure catecholase activity?
    By measuring color change in reaction mixtures
  27. How does Spectronic 20 measure color changes?
    By shining light thru reactants in a test tube & measuring mat of light that penetrates thru the tube
  28. What is transmission on the spectrophotometer?
    How much light penetrates the tube
  29. What is absorption when speaking of the spectrophotometer?
    How much light is being absorbed by the sample
  30. WHat is the relationship between product formation & absorbance?
    As absorbance ↑ so does product formed