Diffusion & Emzyme Lab
Card Set Information
Diffusion & Emzyme Lab
BI 253 Lab
BI 253 Diffusion & Enzyme Lab
A plasma membrane allowing water to pass freely through but regulating mvmt of solvents is called ___ ___.
Water is a universal dissolving agent making it the ____ in a solution.
The substances that are dissolved in a solution are the ____.
What is the physical process where molecules move fr an area of high concentration to an area of low?
What is osmosis?
Diffusion of water thru selectively permeable membrane fr area of high concentration to an area of lower concentration.
Solutes that cannot cross a membrane are called ___ ___.
A hypertonic solution is one in which . . .
Solute concentration is higher outside the cell than inside giving it a greater osmolarity
A hypertonic solution causes water to . . .
Flow out of the cell & eventually crenate
A solution w/greater concentration of solute inside the cell (lower osmolarity) is called ____.
A hypotonic solution cause water to . . .
Flow into the cell and eventually lysis
What is the term for solute concentration expressed in molarity?
2 solutions that are said to be in equilibrium are called ____.
What form of energy drives diffusion?
Intrinsic molecular kinetic energy
When a protoplast, in a plant cell, shrinks & pulls away fr the cell wall due to being placed in a hypertonic solution ____ occurs.
A plant cell exclusive of the cell wall is called a ____.
When a plant cell is placed in hypotonic solution, water flows in expanding the protoplast against the cell wall causing what?
What is water potential in plant cells?
Combined force created by solute concentration & physical pressure
A plant cell that has undergone plasmolysis is said to be ____.
Lg particles suspended in water in motion is traditionally called ___ ___.
What are most enzymes made up of?
pH is a measure of what?
H⁺ in solution
How does salt affect an enzyme?
Like pH there is an optimal salt level
The enzyme catecholase catalyzes a reaction where ___, the substrate becomes ___ (product).
What does benzoquinone look like?
What instrument can we use to measure how much color change happens during a reaction?
How does a Spectronic 20 measure catecholase activity?
By measuring color change in reaction mixtures
How does Spectronic 20 measure color changes?
By shining light thru reactants in a test tube & measuring mat of light that penetrates thru the tube
What is transmission on the spectrophotometer?
How much light penetrates the tube
What is absorption when speaking of the spectrophotometer?
How much light is being absorbed by the sample
WHat is the relationship between product formation & absorbance?
As absorbance ↑ so does product formed