microbio 102 lab final

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jujeebe
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180130
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microbio 102 lab final
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2012-11-01 04:41:28
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lab microbio
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microbio 102 lab final
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  1. TSA: media?
    Tryptic Soy Agar
  2. TSA (tryptic soy agar), test?
    general purpose media - routine cultivation of bacteria
  3. TSA 
    differential, selective?
    neither
  4. TSB, media? test?
    • tryptic soy broth
    • general purpose media - routine cultivation of bacteria 
  5. SAB, media?
    Sabouraud Agar
  6. SAB, test?
    general purpose media- routine cultivation of fungi
  7. SAB, selective or differential?
    selective
  8. BAP, stands for? 
    blood agar plate
  9. BAP, selective or differential?
    Differential
  10. BAP, test?
    production of hemolysins test
  11. Thioglycollate broth, used for?
    has ingredients that bind free O2 = anerobic environment suitable for anaerobic bacteria
  12. Thioglycollate broth, selective or differential?
    Differential 
  13. Motility media, test?
    test for general motility
  14. motility media, selective or differential?
    differential 
  15. motility media, positive test?
    motile organisms will spread through agar and produce diffuse cloudiness = "christmas tree" growth pattern
  16. Fermentaton broth, what and components?
    fermentation of carbohydrates
  17. Fermentation broth, pH indicator?
    phenol red (changes to yellow under acidic conditions)
  18. Fermentation broth, positive result?
    bright YELLOW, (due to acidic conditions)
  19. Fermentation broth, selective or differential?
    Differential media - differentiates  for ability to ferment carbs
  20. what is durham tube?
    added to detect CO2 production
  21. 7% NaCl Agar, made of?
    TSA + 7% sodium chloride
  22. 7% NaCl Agar, positive result?
    GROWTH
  23. 7% NaCl Agar, selective or differential? for what?
    selective media - selective for gram + bacteria
  24. EMB agar, stands for?
    Eosin Methylene Blue Agar
  25. Eosin Methylene Blue Agar (EMB Agar), purpose?
    fermentation of lactose; isolation and differentiation among members of the Enterobacteriaceae
  26. EMB agar, selective or differential?
    BOTH selective and differential media
  27. EMB agar, positive test?
    lactose postitive - colonies are black or black centered with a colorless or pink outer margin
  28. Simmons citrate agar
    • test: citrate utilization test
    • both selective and differential
    • pH indicator: bromthymol blue
    • +: agar changes green -> blue 
  29. Pseudomonas Agar F and P
    • Identification of Pseudomonas Test
    • F: differential, positive - lime green pigment
    • P: differential, positive - blue/green pigment
  30. OF Basal Media with Glucose
    • Oxidation-Fermentation Test
    • DIFFERENTIAL
    • pH indicator: bromthylmol blue
    • positive: OX = open tube yellow, FERM = both tubes yellow

    Purpose of test: differentiates nonenteric gram - bacteria from enteric 
  31. Phenylalanine Agar
    • Phenylalanine Test
    • differential
    • +: green color on slant
    • reagent: ferric chloride soln
  32. TSI (triple sugar iron) Agar
    • Hydrogen Sulfide Production Test
    • differential
    • pH indicator: phenyl red
    • +: black color
  33. Decarboxylation broth
    • Decarboxylation of Amino Acids Test
    • differential
    • pH: bromcresol purple
    • +: purple WITH turbidity 
  34. Starch Agar
    • Starch hydrolysis test
    • differential 
    • +: zone of hydrolysis 
    • reagent: iodine 
  35. skim milk agar
    • casein hydrolysis test
    • differential 
    • +: zone of hydrolysis
  36. DNAse Test Agar
    • DNAse Test
    • differential
    • +: clearing of dye surrounding growth

    purpose of test: differentiates staphlococcus aureus from nonpathogenic staphylococci
  37. Tryptone broth
    • Indol Test
    • differential
    • +: top layer of broth = red
    • reagent: Kovac's solution
  38. MR/VP Broth
    • MR-VP Test (IMViC test)
    • differential 
    • Methyl Red Test
    • ->reagent/ph indicator: methyl red
    • ->positive: RED
    • Voges-Proskauer Test
    • ->reagent: VP reagent A, VP reagent B
    • ->positive: pink/red
  39. Mannitol Salt Agar
    • Mannitol Fermentation Test
    • both selective and differential
    • pH: phenol red
    • +: yellow colonies
  40. Oxidase Test
    • no media, uses Oxistrips 
    • N/A = not selective or differential 
    • +: oxistrip turns blue/black within 20 sec
    • reagent: Kovac's solution

    purpose of test: separate family Pseudomonadaceae from family Enterobacteriaceae
  41. Catalase Test
    • no media
    • N/A = not selective or differential 
    • +: immediate bubbling
    • reagent: 30% hydrogen peroxide 

    • purpose of test: (2)
    • - Streptococcus from Micrococcus and/or Staphylococcus
    • - Bacillus from Clostridium
  42. Coagulase Test
    • no media, citrated rabbit plasma
    • N/A = not selective or differential 
    • +: solid
  43. Both Selective and Differential (3)
    • EMB agar
    • Simmons Citrate Agar
    • Mannitol Salt Agar
  44. Only Selective Media (2)
    • SAB (Sabouraud Agar)
    • 7% NaCl Agar
  45. Neither Selective or Differential (5)
    • 1. TSA
    • 2. TSB
    • 3. Oxidase Test
    • 4. Catalase Test
    • 5. Coagulase Test
  46. pH indicator: bromthymol blue (2 media)
    • simmons citrate agar
    • OF basal media w/ glucose
  47. pH indicator: Phenol Red (3 media)
    • fermentation broth
    • TSI agar
    • Mannitol Salt Agar
  48. pH indicator: bromcresol purple (1 media)
    Decarboxylation broth

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