Lymphoid Tissue Cytology

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kris10leejmu
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181110
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Lymphoid Tissue Cytology
Updated:
2012-11-01 20:55:52
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Lab Tech ll
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Lab Tech ll
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  1. Where is lymphoid tissue found?
    found thoughout the body in nodes, tonsils, and intestines
  2. What is the function of lymphoid tissue?
    filter blood and lymph
  3. What does lymphoid tissue function as?
    • humoral immunity
    • responds to many insult in the body
  4. What is lymphadenopathy?
    • enlarged lymph node
    • lymph gland disease
  5. When do lymph nodes enlarge?
    • primary or systemic disease
    • inflammation
    • hyperplasia
    • first degree neoplasia and second degree neoplasia
  6. Which lymph nodes do we palpate?
    • submandibular
    • axillary
    • prescapular
    • inguinal
    • popliteal
    • mesenteric
  7. How do we make a node aspirate?
    • same as general cytology
    • make 2 smears
    • stain one
  8. How do we do an impression smear of a node?
    • remove node
    • cut open
    • touch slide (normal area and abnormal area)
  9. Are segs normally seen in a lymph node cytology?
    no
  10. _____% of cells are _____.
    • 90
    • small lymphs
  11. Other than small lymphocytes, what else will we see on the cytology?
    • prolymphs
    • lymphoblasts
    • plasma cells
    • macrophages
  12. Describe small lymphocytes (size, shape, cytoplasm, nucleus, chromatin).
    • 10 u
    • round
    • thin rim of blue cytoplasm
    • round nucleus
    • clumpy chromatin
  13. Describe prolymphocytes (nucleus shape, chromatin, cytoplasm).
    • nucleus round or my be indented
    • chromatin less clumpy than lymphocyte
    • more and paler bue cytoplasm than lymphocyte
  14. Describe lymphoblasts (size, cytoplasm, nucleus, chromatin, nucleoli).
    • 15 - 25 u
    • abundant of basophilic cytoplasm
    • large nucleus
    • lacey chromatin
    • 1 - 3 nucleoli
  15. Describe a plasma cell (size, cytoplasm, nucleus, chromatin).
    • medium size
    • moderate to abundant deep blue cytoplasm
    • clumpy chromatin
    • perinuclear clear zone
  16. What do mott cells do?
    make antibodies
  17. Describe a macrophage (size, cytoplasm, nucleus)
    • large cell
    • abundant pale blue cytoplasm with vacuoles
    • pale vesicular nucleus
  18. Is an enlarged node normal or abnormal?
    abnormal
  19. Whast could cause an enlarged node?
    • hyperplasia
    • inflammation
    • neoplasia
  20. What types of inflammation could cause an enlarged node?
    • acute
    • eosinophilic
    • chronic
  21. What are the two types of neoplasia in a node?
    • primary
    • metastatic
  22. What does a cytology of a hyerplasia lymph node look like?
    • small lymphs (>70% seen)
    • numerous plasma cells
    • increase in prolymphs, blasts, and macrophages
    • may see mast cells, eosinophils, few sges, mott cells
    • organisms may be present
  23. Where would there be hyperplasia in a lymph node?
    immune system stimulated for some reason
  24. Do the lymphocytes look normal on a cytology of hyperplasia of a lymph node?
    yes
  25. If there are predominately segs on a cytology of lymphadenitis, then what is it?
    purulent inflammation
  26. If there are more eosinophils on a cytology of lymphadenitis, then what is the cause?
    allergy or parasite
  27. If there are more macrophages on a cytology of lymphadenitis, then what is the cause?
    • fungi
    • foreign body
  28. Where do we look for the etiologic agent (organisms) in lymphadenitis?
    • in cytoplasm of segs and macrophages
    • between cells
  29. What is another term for primary neoplasia?
    lymphoma
  30. What species is lymphoma common in and what else do we see with the patients who have lymphoma?
    • dogs and cats
    • hypercalcemia (elevated calcium levels)
  31. If there are many lymph nodes enlarged without dermatitis then what should we suspect it is?
    lymphoma
  32. What does a cytology look like for lymphoma?
    • monomorphic population of large lymphs
    • plasma cells are rare
    • few small lymphs
  33. What are the different types of lymphoma?
    • lymphoblastic
    • prolymphocytic
    • histiocytic
    • lymphocytic
  34. Which type of lymphoma is the most common?
    lymphoblastic
  35. Which type of lymphoma is the rarest?
    lymphocytic
  36. Who determines the type of lymphoma?
    pathologist
  37. We don't often see "malignant criteria" for _____.
    lymphoma
  38. What is secondary neoplasia?
    metastasis
  39. What does a cytology look like of metastasis?
    • large cells with neoplastic criteria
    • cells may be unrecognizable
  40. What is the difference between primary neoplasia (lymphoma) and secondary neoplasia (metastasis)?
    • primary neoplasia (lymphoma) starts in the lymph nodes
    • secondary neoplasia starts somewhere else and metastasizes to the lymph nodes
  41. What does the normal cytology of a spleen look like?
    • similar to a normal node...
    • except 75% small lymphs
    • increased prolymphs, blasts, and plasma cells
    • many rbcs, obsure cells
  42. Why would we do a cytology of the spleen?
    splenomegaly

  43. What is this?
    acute inflammation in the lymph node

  44. What is this?
    • blastomyces
    • organism in acute inflammation

  45. What is this?
    • cryptococcosis
    • organism in acute inflammation

  46. What is this?
    • histoplasma
    • organism in acute inflammation

  47. What is this?
    hyperplasia in a lymph node

  48. What is this?
    hyperplasia in a spleen

  49. What is this?
    • leishmania
    • organism in acute inflammation

  50. What are the two large cells?
    lymphoblasts

  51. What is wrong with this animal?
    lymphoma

  52. What is wrong with this animal?
    lymphoma

  53. What are the large cells?
    macrophages

  54. What is this?
    mast cell tumor

  55. What cells are these?
    Mott cells

  56. Is this cytology of a lymph node normal or abnormal?
    normal

  57. What cell is this?
    plasma cell

  58. What cell is the arrow pointing to?
    prolymphocyte

  59. What type of neoplasia is this?
    secondary neoplasia

  60. What organ is this a cytology of?
    the spleen

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