micro test3 3rd

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sullydog101
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micro test3 3rd
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2012-11-10 19:50:09
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micro test3 3rd
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  1. What is the oxidative classification of Enterobacteriaceae?
    Oxidase negative – this is a distinguishing characteristic
  2. What disease is associated with EPEC?
    • EPEC – Infant Diarrhea
    • ETEC – Travelers Diarrhea, Infant Diarrhea
    • EHEC – Bloody Diarrhea, Hemorrhagic colitis, HUS
    • EIEC – Bacillary dysentery
    • EAEC – Infant diarrhea, traveler’s diarrhea
    • DAEC – Infant diarrhea
  3. How does EPEC cause disease?
    • 1. Loose attachment with bundle forming pili
    • 2. Type III secretion of translocated intimin receptor (Tir)
    • 3. Tir dimerizes and locates to host membrane
    • 4. Intimin binds to Tir
    • 5. microvilli undergo effacement and do no absorb effectively
  4. What disease is associated with ETEC?
    • EPEC – Infant Diarrhea
    • ETEC – Travelers Diarrhea, Infant Diarrhea
    • EHEC – Bloody Diarrhea, Hemorrhagic colitis, HUS
    • EIEC – Bacillary dysentery
    • EAEC – Infant diarrhea, traveler’s diarrhea
    • DAEC – Infant diarrhea
  5. What treatment is for ETEC?
    Usually just fluids and rest, no antibiotic!
  6. How does ETEC cause diarrhea?
    • Two toxins: heat stable and heat labile
    • Heat labile (LT-1) has 5 binding (B) parts and one A part
    • B parts bind to cell and ins endocytosed, where A subunit leaves
    • A subunit transfers ADP-ribose from NAD to GDP of Gs which increases cAMP
    • Increased cAMP increases chloride secretion and decreases sodium absorption
    • Heat stable toxin (STa) is small and monomeric (can’t be heat stable if too big)
    • It binds to hist guanylyl cyclase which catalyzes GTP to cGMP which stimulates secretion of chloride
    • Both of these work through the CFTR
  7. What disease is associated with EHEC?
    • EPEC – Infant Diarrhea
    • ETEC – Travelers Diarrhea, Infant Diarrhea
    • EHEC – Bloody Diarrhea, Hemorrhagic colitis, HUS
    • EIEC – Bacillary dysentery
    • EAEC – Infant diarrhea, traveler’s diarrhea
    • DAEC – Infant diarrhea
  8. How does EHEC cause disease?
    • Shiga toxin, Stx-1 and or Stx-2
    • Cause A/E lesion on epithelial cells
    • Large plasmid transferred by bacteriophages
    • Both toxins are A-B toxins with 1 A and 5 B
    • Stx-1 is same toxin produced by Shigella dysenteriae
    • Stx can transfer across epithelial cells and enter bloodstream
    • B subunits bind to GB3 (globotriaosylceraminde) on endothelial cells
    • A subunit then binds to 28S sRNA of the 60S unit disrupting protein synthesis and causing cell death
    • Coagulation occurs in the microvasculature, platelets are consumed, renal failure
  9. What disease is associated with EIEC?
    • EPEC – Infant Diarrhea
    • ETEC – Travelers Diarrhea, Infant Diarrhea
    • EHEC – Bloody Diarrhea, Hemorrhagic colitis, HUS
    • EIEC – Bacillary dysentery
    • EAEC – Infant diarrhea, traveler’s diarrhea
    • DAEC – Infant diarrhea
  10. How does EIEC cause disease?
    • Invasion and destruction of colonic epithelium.
    • Large plasmid carries the virulence factors (ipa)
    • Bacteria become internalized in phagolysosome and escape into cytoplasm
    • Spread to neighboring cells via host actin tails
  11. What disease is associated with EAEC?
    • EPEC – Infant Diarrhea
    • ETEC – Travelers Diarrhea, Infant Diarrhea
    • EHEC – Bloody Diarrhea, Hemorrhagic colitis, HUS
    • EIEC – Bacillary dysentery
    • EAEC – Infant diarrhea, traveler’s diarrhea
    • DAEC – Infant diarrhea
  12. How does EAEC cause disease?
    • EAEC agglutinate into stacked brick arrangement by fimbriae I and II
    • EAEC stimulates mucous secretion, which traps bacteria in biofilm
    • Microvilli shorten, mononuclear cells infiltrate, and hemorrhage occurs
    • Chronic diarrhea in children in developing countries associated with growth retardation
  13. What disease is associated with DAEC?
    • EPEC – Infant Diarrhea
    • ETEC – Travelers Diarrhea, Infant Diarrhea
    • EHEC – Bloody Diarrhea, Hemorrhagic colitis, HUS
    • EIEC – Bacillary dysentery
    • EAEC – Infant diarrhea, traveler’s diarrhea
    • DAEC – Infant diarrhea
  14. How does DAEC cause disease?
    Diffuse attachment of bacteria to epithelium by D.R. like fimbrial adhesin
  15. What strain of E. Coli can be detected easily in local lab?
    • EHEC
    • All others must be sent off to reference laboratories
    • EHEC culture on MacConkey agar and then assay for toxin
  16. What do you treat E. Coli with?
    • UTI: cephalosporins, trimethoprim, sulfamethoxazole, sometimes nalidixix acid or tetracylcline
    • Diarrhea or hemorrhagic colitis: supportive therapy (fluids, rest)
    • HUS: IV fluids and nutrition, blood transfusions, dialysis

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