What are the eight steps?
- 1. Acetyl CoA adds two- carbon acetyl to oxaloacetate, forming citrate
- 2. Citrate converted to isocitrate by removal of one water molecule and addition of another
- 3. Isocitrate is oxidized by NAD+, which becomes NADH. CO2 is lost
- 4. Another CO2 is lost and the resulting compound is oxidized, reducing NAD+.
- 5. CoA is displaced by a phosphate group, which is transferred to GDP, forming GTP
- 6. Two hydrogens are transferred to FAD, forming FADH2
- 7. Addition of a water rearranges substrates’ bonds
- 8. Substrate is oxidized, reducing NAD+, regenerating oxaloacetate