Mycology Lab

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kris10leejmu
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183496
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Mycology Lab
Updated:
2012-11-17 19:37:33
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Lab Tech ll Practical
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Lab Tech ll
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  1. What does cryptococus neoformans cause?
    cryptococcosis
  2. Where in the body do we find cryptococus neoformans?
    • CNS
    • lungs
    • lymph nodes
  3. What does Aspirgillus fumigatus cause?
    aspergillosis
  4. What does Aspirgillus fumigatus do to ruminants?
    causes abortions
  5. Where can we find Aspirgillus fumigatus in the body?
    • lungs
    • nasal passages
  6. Which species is Aspirgillus fumigatus a problem in?
    birds
  7. Histoplasma capsulatum is a _____ fungi.
    dimorphic (mold and yeast forms)
  8. What does Histoplasma capsulatum cause?
    histoplasmosis
  9. Where in the body do we find Histoplasma capsulatum?
    lungs and lymph nodes
  10. Where in the environment do we find Histoplasma capsulatum?
    river valleys
  11. Where should we look for Histoplasma capsulatum on a cytology?
    in macrophages
  12. Are there different tests available for Histoplasma capsulatum?
    yes, ELISA/skin tests
  13. Blastomyces dermatitidis is a _____ fungi.
    dimorphic (mold and yeast forms)
  14. What does Blastomyces dermatitidis cause?
    blastomycosis
  15. Where in the US and environment do we find Blastomyces dermatitidis?
    Mississippi and Ohio river valleys
  16. Where in the body do we find Blastomyces dermatitidis?
    • lungs
    • skin
    • lymph nodes
  17. Blasomyces dermatitidis causes what kind of inflammation?
    chronic
  18. Other than cytology, what other tests are available for Blastomyces dermatitidis?
    ELISA/skin tests
  19. What is the normal flora of the GI and urinary tract?
    Candida albicans
  20. When we have an overgrowth of Candida albicans what do we call it?
    • thrush
    • frounce
  21. Which species does Candida albicans become pathogenic in?
    birds
  22. What is a common contaminate that grows on DTM plates?
    • alternaria
    • common environmental fungus found in soil, plants, food and indoor air
    • when cultured, the colonies are black due to the production of melanin - like pigments (this is why tomatoes turns black)
  23. Coccidiodes immitis is a _____ fungi.
    dimorphic (mold and yeast forms)
  24. What does Coccidiodes immitis cause?
    coccidiodomycosis (valley fever)
  25. Where in the US do we find Coccidiodes immitis?
    dry areas (south west US)
  26. Where in the body do we find Coccidiodes immitis?
    lungs but could also spread to other sites like bone
  27. Does Coccidiodes immitis bud?
    no
  28. When we are looking at Coccidiodes immitis, how do we focus with the microscope?
    in focus with neutrophils
  29. What is another name for Microsporum gypseum?
    ringworm
  30. Microsporum gypseum is part of the _____ mycosis.
    superficial
  31. Is Microsporum gypseum a dermatophyte?
    yes
  32. Can we culture Microsporum gypseum in house?
    yes, on DTM
  33. Is Microsporum canis a dermatophyte?
    yes
  34. Microsporum canis is part of the _____ mycosis.
    superficial
  35. What is another name for Microsporum canis?
    ringworm
  36. Can we culture Microsporum canis in house?
    yes, on DTM
  37. Is Trichophyton mentagrophytes a dermatophyte?
    yes
  38. What is another name for Trichophyton mentagrophytes?
    ringworm
  39. How is Trichophyton mentagrophytes spread?
    by direct contact
  40. Other than mycellium, what else can cause a color change on a DTM?
    bacteria and other contaminants
  41. What do we use 10% potassium hydroxid (KOH) for?
    used as a clearing agent to remove debris when examining hair shafts microscopically
  42. What are arthrospores within the hair shaft called?
    endothrix
  43. What are arthrospores outside the hair shaft called?
    ectothrix
  44. What is the technique called when we use KOH to remove arthrospores?
    trichogram
  45. A positive result with the wood's lamp indicates _____.
    Microsporum canis
  46. What does a positive result for Microsporum canis look like under a wood's lamp?  What does dandruff look like?
    • Microsporum canis:  apple green glow
    • Dandruff:  white
  47. How long does it take for a wood's lamp to warm up?
    5 minutes
  48. Cryptococcus neoformans

    • single, large, thick walled yeast
    • surrounded by a non-staining, wide gelatinous capsule
    • may see budding forms
  49. Cryptococcus neoformans

    • single, large, thick walled yeast
    • surrounded by a non-staining, wide gelatinous capsule
    • may see budding forms
  50. Aspirgillus fumigatus

    • hyphae and spores most often seen
    • cultures may exhibit conidiophores (fruiting) bodies
  51. Aspirgillus fumigatus

    • hyphae and spores most often seen in clinical samples
    • cultures may exhibit conidiophores (fruiting) bodies
  52. Histoplasma capsulatum

    • small yeast form
    • thick wall
    • often fills macrophages
    • often budding
  53. Histoplasma capsulatum

    • small yeast form
    • thick wall
    • often fills macrophages
    • often budding
  54. Blastomyces dermatitidis

    • large yeast form
    • thick, double wall
    • basophilic with Diff-Quik stain
    • often budding
  55. Blastomyces dermatitidis

    • large yeast form
    • thick, double wall
    • basophilic with Diff-Quik stain
    • often budding
  56. Candida albicans

    • yeast cells occasionally in strings (pseudohyphae)
    • true short hyphae may be present
  57. Candida albicans

    • yeast cells occasionally in strings (pseudohyphae)
    • true short hyphae may be present
  58. Alternaria

    • common environmental fungus found in soil, plants, food, and indoor air
    • often considered an environmental contaminant in DTM cultures
  59. Coccidiodes immitis

    • mold form - barrel shaped arthrospores
    • arthrospores are the infectious form
  60. Coccidiodes immitis

    yeast form - huge spherules that contain numerous small endospores
  61. Microsporum gypseum

    • rounded ends with no knobs
    • rowboat shaped
    • fewer than 6 cells
    • thin walled macroconidia
  62. Microsporum gypseum

    • rounded ends with no knobs
    • rowboat shaped
    • fewer than 6 cells
    • thin walled macroconidia
  63. Microsporum canis

    • knob seen on terminal end
    • canoe shaped
    • 6 or more cells
    • thick walled macroconidia
  64. Microsporum canis

    • knob seen on terminal end
    • canoe shaped
    • 6 or more cells
    • thick walled macroconidia
  65. Trichophyton mentagrophytes

    • cigar shaped
    • thin walls
    • variable number of cells inside
  66. Trichophyton mentagrophytes

    • cigar shaped
    • thin walls
    • variable number of cells inside

  67. What type of DTM is this?
    Fungassay
  68. How do we do a microscopic observation of hair shafts?  What do we look for?  What is endothrix? What is exothrix?
    • Add KOH to the hair shaft on the slide, gently heat
    • look for arthrospores - asexual, thick walled spores formed by hyphal separation
    • endothrix:  within the hair shaft
    • exothrix:  outside of the hair shaft
  69. What do we need to pay attention to when we are observing fungi under the microscope?
    • size of organism
    • thickness of the cell wall
    • structures and cells surrounding the organism
    • staining of organisms and surrounding areas
  70. When we are staining a tape sample of skin, which Diff Quik stains do we skip and why?
    the fixative because the alcohol in the fixative will eat the adhesive from the tape
  71. How do we do a microscopic observation after a DTM?
    • place a drop or two of LactoPhenol Cotton Blue stain on a slide
    • gently tease a small amount of growth from the surface of the agar and gently mix with the stain on the slide
    • add a cover slip and observe under 10x and 40x
  72. What stain do we use to view organisms after a DTM?
    LactoPhenol Cotton Blue
  73. What is the most distinguishing characteristic of Blastomyces?
    very large
  74. What is the main distinguishing characteristic of the Cryptococcus organism?
    single large thick walled yeast surrounded by non-staining wide gelatinous capsule
  75. Describe the difference between microconnidia and macroconnidia?
    macroconnidia is larger than microconnidia
  76. Besides the ear, what other areas of the body are often affected by Malassezia in dogs?
    skin

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