Lymph Node Cytology Lab

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kris10leejmu
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183723
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Lymph Node Cytology Lab
Updated:
2012-11-14 21:18:16
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Lab Tech ll Practical
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Lab Tech ll
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  1. What is hyperplasia?
    the increased population of normal cells
  2. Which cell is the most numerous cell seen with spleen hyperplasia?
    small lymphs (> 70%)
  3. In addition to the most numerous cell being the most in spleen hyperplasia, what other cells could we see?
    • plasma cells
    • increase in prolymphs, blasts, and macrophages
    • occasional mott cell may be present
  4. Are eosinophils normally found in normal lymph node tissue?
    no
  5. What is eosinophilic lymphadenitis?
    eosinophils in lymph node tissue
  6. What does the tissue look like grossly with hyperplaisa?
    tissue is enlarged
  7. What does lymphoma look like on a cytology?
    large, immature lymphoid cells
  8. In addition to large, immature lymphoid cells seen with lymphoma, what else could we see on a cytology?
    lymphoglandular bodies
  9. What are lymphoglandular bodies?
    • resemble platelets in size and staining characteristics, but they are fragments of cytoplasm due to the destruction of fragile cells
    • in addition, the lyzed nuclei of these cells may stain as lacy amorphous eosinophilic material
  10. Can we see lymphoglandular bodies in other conditions besides lymphoma?
    yes, we can see them in other conditions having fragile cells
  11. What kind of neoplasia is lymphoma?
    discrete cell neoplasia
  12. Does the typical neoplastic criteria apply when concluding that a sample is suggestive of lymphoma?
    no
  13. If a sample exhibits a percentage of immature lymphs greater than _____, it is lymphoma.
    50%
  14. Is cutaneous lymphoma common in dogs and cats?
    no
  15. Is lymphoma restricted to just lymphoid tissue?
    no
  16. What is inflammation of a lymph node called?
    lymphadenitis
  17. Is lymphadenitis routinely seen in dogs and cats?
    yes
  18. What type of lymphadenitis is it if the predominate cell is neutrophils?
    purulent inflammation
  19. What kind of lymphadenitis is it if the predominate cell is eosinophils?
    allergy or parasites
  20. What does increased macrophages in lymphadenitis suggest?
    fungi or foreign body
  21. What does the cytology of a normal lymph node look like?
    • 90% small lymphs
    • presence of prolymphs, lymphoblasts, plasma cells and macrophages are normal
  22. How do we know if secondary neoplasia is going on in a lymph node?
    • finding large cells with neoplastic criteria within the lymph node suggests metastasis from another site
    • these cells may be unrecognizable¬†
  23. What does a cytology of lymph node hyperplasia look like?
    >70% of small lymphs
  24. Since hyperplasia of a lymph node is an increase population of lymphs but a normal lymph node cytology also looks like this, what makes hyperplasia easier to diagnose?
    the lymph node will be enlarged...a normal node will not be enlarged
  25. In addition to small lymphs in lymph node hyperplasia, what else might we see on the cytology of lymph node hyperplasia?
    • plasma cells
    • increase in prolymphs, lymphoblasts, and macrophages
    • occasional mott cells
  26. Is cutaneous lymphoma common in dogs and cats?
    no
  27. spleen hyperplasia

    • >70% small lymphs
    • increase in prolymphs, lymphoblasts, macrophages
    • mott cells may also be present
  28. eosinophilic lymphadenitis
  29. lymphoma

    more than 50% of lymphs are large, immature lymphocytes

  30. What is the stuff in the backgroud of the lymph nodes?
    lymphoglandular bodies

    resemble platelets in size and staining characteristics but they are fragments of cytoplasm due to the destruction of fragile cells
  31. lymphoma
  32. lymphoma
  33. lymphoma
  34. lymphoma
  35. normal lymph node

    • 90% cells are small lymphs
    • presence of prolymphs, lymphoblasts, plasma cells, and macrophages are normal

  36. What kind of neoplasia is this from a lymph node
    secondary neoplasia

    • large cells with neoplastic criteria within the lymph node
    • cells may be unrecognizable as to their origin

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