Mech of neurotransmitter release

Card Set Information

Author:
mfawcett
ID:
185242
Filename:
Mech of neurotransmitter release
Updated:
2013-01-13 05:24:42
Tags:
MODA SNARE neurotransmitter release
Folders:

Description:
mech of neurotransmitter release
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user mfawcett on FreezingBlue Flashcards. What would you like to do?


  1. Visualising nerve junctions
    Make fluorescent derivative of NA with hot formaldehyde vapour
  2. Describe SNAREs
    • Synaptobrevin: v-SNARE, anchored in vesicle membrane by a hydrophobic carboxy terminus. central 70aa alpha-helix
    • Syntaxin: t-SNARE
    • SNAP 25: t-SNARE
    • Core complex: 4 a-helices (1 each, 2 from SNAP 25)
    • form a leucine zipper -> brings vesicle and plasma membranes close together
  3. Botulinum toxin
    • Cleaves target SNARE by peptidase activity
    • Specific SNARE depends on which of 7 subtypes
    • B, D, F, G: Synaptobrevin
    • A, E: SNAP-25
    • C1: Syntaxin and SNAP-25
    • Insertion into cholinergic neurons: C-terminus of heavy chain binds nucleoside receptor eg GT1b, N terminus translocates light chain in through channel
    • Botulinum anti-toxin only works within 30mins of adsorption of toxin
    • Symptoms: somatic muscle weakness (inc resp), loss of cholinergic activity (constipation, blurred vision, dry skin, urinary retention), slowed heart rate (due to effects on NA nerves?)
  4. Tetanus toxin
    • Binds to synaptobrevin
    • Retrogradely transported to cell body, then transferred to inhibitory interneuron - this cannot now release its transmitter, so the motor neuron becomes excitable
  5. Synaptotagmin
    • Calcium sensor
    • N-terminal TM region in vesicle
    • 2 low affinity Ca binding regions (homology to PKC)
    • binds to phospholipids (inc syntaxin) in Ca dependent manner
  6. Synapsins
    • Non-phosphorylated: bound to vesicles and cytoskeleton
    • Phosphorylated (by PKA and CaM kinase II): dissociate from vesicle: vesicle can translocate to active zone

What would you like to do?

Home > Flashcards > Print Preview