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Explain various denaturation/renaturation processes associated w/ DNA
- Molecular hybridization: renaturing two DNAs or DNA:RNA from different sources
- Fluorescent in situ Hybridization (FISH): fluorescent ssDNA or RNA is used as a probe to identify specific chromosomal locations of genetic information
- Reassociation Kinetics and repetitive DNA: DNA is fragmented and dissociated, then allowed to reassociate. Can show/explain various levels of repetitive DNA in a genome. (more pieces [more repeats]= faster reassociation)
Define hyperchromatic shift, melting temperature
- Hyperchromatic shift: the increase in the UV absorption as DNA denatures from ds to ss
- Melting temperature: the point at which 50% of the DNA strands are unwound or denatured for a given piece of DNA
Describe the three distinct classes of RNA sequences that make up eukaryotic organisms
- rRNA: structure components of ribosomes
- mRNA: carry genetic information from DNA to ribosome
- tRNA: carries amino acids to ribsome during translation
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