GeneticsCh10pt2

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Author:
victimsofadown
ID:
186018
Filename:
GeneticsCh10pt2
Updated:
2012-11-29 04:17:53
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GeneticsTest3
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GeneticsTest3
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  1. Explain various denaturation/renaturation processes associated w/ DNA
    • Molecular hybridization: renaturing two DNAs or DNA:RNA from different sources
    • Fluorescent in situ Hybridization (FISH): fluorescent ssDNA or RNA is used as a probe to identify specific chromosomal locations of genetic information
    • Reassociation Kinetics and repetitive DNA: DNA is fragmented and dissociated, then allowed to reassociate.  Can show/explain various levels of repetitive DNA in a genome.  (more pieces [more repeats]= faster reassociation)
  2. Define hyperchromatic shift, melting temperature
    • Hyperchromatic shift: the increase in the UV absorption as DNA denatures from ds to ss
    • Melting temperature: the point at which 50% of the DNA strands are unwound or denatured for a given piece of DNA
  3. Describe the three distinct classes of RNA sequences that make up eukaryotic organisms
    • rRNA: structure components of ribosomes
    • mRNA: carry genetic information from DNA to ribosome
    • tRNA: carries amino acids to ribsome during translation

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