What are the differences between these vectors, and what hosts would they be introduces to? plasmid, YAC, BAC, HAC, Ti Plasmid, Cosmid
- Plasmid: introduced to bacteria via transformation. Plamsid and DNA are cut with same restriction enzyme, then allowed to anneal. Many copies per bacteria. Blue-white selection is used to determine which bacteria have the desired product. Too small for some larger genes.
- YAC: yeast artificial chromsomes. Can clone large-fragments of DNA. Linear, has telomers, origins or rep, centromere.
- BAC: bacterial articificial chromsomes. Large, but low copy (1-2 per cell) plasmids.
- Ti Plasmid: Plants. Plasmid recovered from Agrobacterium. Segment of DNA from plasmid is transfered to plant cell genome. Tumor-causing elements removed from T-DNA.
- Cosmid: Created from plasmid AND virus components.