bio ch 12

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lexdelmonico
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bio ch 12
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2012-12-02 15:50:52
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bio ch 12
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  1. What did Frederick Griffith learn?
    how certain types of bacteria caused pneumonia
  2. Griffith made 2 observations, what were they?
    • 1. The disease-causing S-strain (virulent) strain of bacteria grew into smooth colonies o nculture
    • 2. The harmless strain (R-strain) grew into colonies with rough strains
  3. How many experiments did Griffith do?
    four
  4. What did Griffith do in experiment 1?
    mice injected with disease-causing (virulent) strain of bacteria, mice got pneumonia= mice died
  5. What did Griffith do in experiment 2?
    mice injected with harmless strain of bacteria= mice live
  6. What did Griffith do in experiment 3?
    Griffith heated the disease-causing bacteria, injected the heat-killed bacteria into the mice= mice lived
  7. What did Griffith do in experiment 4?
    heat-killed, isease-causing bacteria + live, harmless bacteria; injected the mixture into the mice = mice got pneumonia and died
  8. What was Griffith's transformation?
    one strain of vacteria (harmless) had changed pemanently into another (disease)
  9. What did Griffith hypothesize?
    a factor must contain info that could change harmless bacteria into disease-causing ones
  10. Who repeated Griffiths work in 1944?
    Oswald Avery
  11. What was the first stage in Avery's experiment, and what was the result?
    destroyed all macromolecules except DNA; transformation still occured
  12. What did Griffith conclude?
    that heat killed bacteria passed their disease causing ability to the harmless strand
  13. What happened whaen Avery destroyed the DNA?
    no transformation occurred
  14. What was Avery's conclusion?
    transfomrming factor in bacteria= DNA
  15. In 1952, who worked with T2 bacteriophages, and what were they? What were the bacteriophages composed of?
    Hershey and Chase; virus that infects and kills bacteria; composed of DNA core with a protein coat
  16. What did Hershey and Chase do in their experiment?
    attaches to bacterium and injects its DNA(viral genes)- makes bacterium a virus factory- "hijacks" cell- produces new T2 bacteriophages and gradually destroys bacterial cells
  17. What was the result of Hershey and Chase experiment?
    the bacterial cell splits open and hundreds of new viruses burst out of host cell
  18. What did Hershey and Chase determine?
    it was the DNA that entered and infected the cell by using radioactive isotopes
  19. What is DNA made up of?
    nucleotides
  20. What are the parts of DNA?
    • sugar (deoxyribose)
    • phosphate
    • nitrogen base
  21. What are the four nucleotides of DNA?
    • adenine
    • guanine
    • thymine
    • cytosine
  22. What are Chargaff's Rules (base pairing rules)?
    • 1. A is found in equal amount to T and C is found in equal amount to G
    • 2.therefore, A=T and C=G
  23. What did Rosalind Franklin do?
    used x-ray diffractioin to take a picture of DNA
  24. What did Watson and Crick do?
    the used Franklins picture of DNA, and found out DNA is a double helix (twisted ladder)
  25. Where are prokaryotes DNA  located?
    cytoplasm
  26. What is single circular DNA?
    one chromosome with several genes
  27. Where are eukaryotes DNA located?
    nucleus
  28. What  are DNA and protein (histones)?
    chromatin
  29. Chromatin form bead like structure called____.
    nucleosome
  30. Nucleosomes help do what/
    fold long DNA to fit into the tiny spaces
  31. What are tightly coiled up chromatin?
    chromosome
  32. What is DNA replication?
    when the DNA molecule produces two complementary strands
  33. DNA replication starts at a single poin (replication fork) and _______.
    proceeds in two different directions
  34. What unwinds and "unzips" DNA?
    helicase
  35. What are broken between base pairs?
    hydrogen bonds
  36. What are the differences between RNA and DNA?
    • single strand of nucleotides
    • sugar is ribose
    • base pairing has uracil instead of thymine
  37. What are the types of RNA?
    messenger, ribosomal, and transfer
  38. What is messenger RNA?
    carries instructions for making proteins from DNA into ribosomes
  39. What is ribosomal RNA?
    makes up ribosomes, helps assembal proteins
  40. What is transfer RNA?
    transfers amino acids to the ribosome
  41. What is transcription?
    making mRNA from DNA
  42. What are the steps of transcription?
    • 1. mRNA makes a copy of DNA- RNA polymerse
    • 2. starts at promoter site on DNA
    • 3. RNA nucleotides pair up with DNA to make mRNA
    • 4. mRNA leaves nucleus: goes to ribosome
  43. what is an exon?
    expressed sequence of DNA- codes for protein
  44. What is an intron?
    interrupting noncoding sequences of DNA- does not code for protein
  45. What are the steps of RNA editing?
    • 1. mRNA strand introns cut out, so only exons left
    • 2. Final mRNA transcrtipt= exon spliced together, cap and tail are added (prevents degradation/ damage on the way to ribosome)
  46. Why is this editing important to organisms?
    • 1. one gene on DNA can be cut and spliced to make different RNA strands for different versions of a protein
    • 2. role in evolution- very small changes in DNA have large effects in gene expresions
  47. What is a codon?
    a sequence of 3 bases on mRNA that codes for a specific amino acid
  48. What is the genetic code?
    3 bases read at a time= a one "word" (one amino acid)
  49. Four different letters used to code for how many amino acids?
    20
  50. How many codons does one amino acid have?
    it can have many
  51. What is the start codon?
    AUG
  52. What are the stop codons?
    UGA, UAA, UAG
  53. WHAT IS TRANSLATION?
    decodin of mRNA into protein (polypeptide chain)
  54. What does translation begin with?
    mRNA attatches to ribosome on start codon
  55. What are the steps of Translation?
    • 1. each codon moves through rivosome and correct amino acid is brought to robosome by tRNA
    • 2.anticodon on tRNA pairs with codon on mRNA
    • 3.peptide bond forms between amino acids
    • 4. mRNA move through until stop codon
    • 5. New protein (polypeptide chain) and mRNA are released from ribosome`
  56. What are anticodon?
    sequence of three bases on tRNA

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