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Order
Cell - Nucleus - Chromosome - DNA - Gene
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Purpose of Genes
Provide the code for the instructions to produce proteins
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First modern biotechnology
Insulin
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Genetic Code
The set of rules by which genetic material is translated into proteins
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RNA Types
- mRNA (messenger)
- tRNA (transfer)
- rRNA (ribosomal)
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Bacteriophage
Virus that reproduces inside of a bacteria
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Genetic Engineering Def:
modification of an organism by manipulation its genetic material
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Recombinant DNA
DNA made in the lab
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Restriction Endonuclease
Enzymes that cut DNA at specific nucleotide sequences
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DNA amplification
production of multiple copies of a DNA sequence
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Polymerase Chain Reaction (PCR)
produce millions of copies of DNA sequence to bypass need to use bacteria
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Kary Mullis
Won Nobel Prize for improving PCR technique
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Taq Polymerase
Bacterium that lives in hot springs - an enzyme able to withstand protein killing conditions at high temperatures.
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Autoradiograph
Used to identify children or heredity DNA fingerprinting
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Pathogen
microorganism that causes disease
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Antigen
Antibody Generator - triggers immune system response to create antibodies specific to the antigen
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Antibody
Immune systems way to identify and neutralize any bad stuff
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Pluripotent
Embryonic - all cell types
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Embryonic Stem Cells
Get from embryos that are a week old or from blood in umbillical cord
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Adult Stem cells
Undifferentiated cells - specialized for certain organs
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Blastocyst
Very early pre-embreo
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Gene Therapy
Using an adenovirus vector, DNA is injected and used to fight a disease
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nonsense mutation
Premature STOP codon in the DNA
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missense mutation
incorrect subtitution nucleotide where incorrect amino acid is produced in the DNA
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Adenovirus
double stranded - common cold virus
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Retrovirus
double stranded - HIV
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monoclonal antibodies
clone antibodies that bond to the same epitope
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hybridoma
a somatic cell hybrid formed by fusion of normal lymphocytes and tumor cells
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ELSI
Ethical, Legal, and Social Issues
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BGH
Bovine Growth Hormone
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Gene Gun
Inserts genes for transformation into plant cells using gold particles
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Transgenic Animals
Currently, the three most widely used procedures for creating transgenic animals are microinjection of the cloned gene(s) into the pronucleus of a fertilized egg, injection of recombinant embryonic stem cells into embryos, and the use of retroviruses.
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Transgenics application
Alzheimers
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genome
entirety of organisms hereditary information
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Chromosome
Single piece of DNA
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Replication
DNA replication is a biological process that occurs in all living organisms and copies their DNA; it is the basis for biological inheritance
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Transcription
DNA transcription is a process that involves the transcribing of genetic information from DNA to RNA.
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Translation
Translation is the process through which cellular ribosomes manufacture proteins, in which messenger RNA (mRNA) is sequentially decoded bytransfer RNA
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Nucleotide
Nucleotides are biological molecules that form the building blocks of nucleic acids (DNA and RNA) and serve to carry packets of energy within the cell (ATP)
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codon
a series of three adjacent bases in one polynucleotide chain of a DNA or RNA molecule, which codes for a specific amino acid.
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plasmid
A plasmid is a small DNA molecule that is physically separate from, and can replicate independently of, chromosomal DNA within a cell
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ribosome
The ribosome is a large and complex molecular machine, found within all living cells, that serves as the primary site of biological protein synthesis.
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totipotent
capable of developing into a complete organism or differentiating into any of its cells or tissues
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pluripotent
not fixed as to developmental potentialities; especially :capable of differentiating into one of many cell types
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multipotent
unspecialized cells that have the ability to: Self-renew for long periods of time and differentiate into specialized cells with specific functions
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insulin
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body.
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down syndrome
is a genetic condition in which a person has 47 chromosomes instead of the usual 46.
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restriction enzyme
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at specific recognition nucleotide sequences known asrestriction sites
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GMO
Genetically Modified Organism
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Pharming
Pharming is a portmanteau of farming and "pharmaceutical" and refers to the use of genetic engineering to insert genes that code for useful pharmaceuticals into host animals or plants that would otherwise not express those genes, thus creating a genetically modified organism (GMO)
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