Bio 2010

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Bio 2010
2012-12-04 19:38:55
Sterilization innate immunity antimicrobials

Bio 2010 chpt. 10
Show Answers:

  1. what does sterilization get rid of
    bacteria, molds/yeasts, viruses, protozoans
  2. who found connection between childbed fever and washing hands after autopsy
    ignaz semmelweis
  3. what was the first to wash dressings with carbolic acid
    joseph lister
  4. what are the times and temps for boiling water, and what is still viable
    • 10 min at 100 degrees C
    • endospores and endotoxins
  5. what is pasteurization used to destroy
    salmonella, brucella, mycobacteria
  6. What is HTST
    high temp short time
  7. what is HTST for pasteurization
    15 sec at 72 degrees c
  8. what is ultra high for pasteurization
    several seconds at 140 degrees C then cool quickly
  9. autoclave pressure, time, and temp?
    15 pounds per square inch above atmosphere pressure, temp 121 C, time 15-20 mins
  10. steps for autoclave?
    • 1. cold air is removed
    • 2. steam enters
    • 3. condensation at 100 C
    • 4. add more steam to increase pressure by 15 pounds per inch squared
    • 5. temp 121 C for 15-20 mins
    • 6. remove steam, replace w/ cool air
  11. Quality control?
    place test container in autoclave, after autoclaving crush ampule w/in container, place in incubator at 60 C for 24 hrs, look for purple/blue colors, bacillus stearothermophilus
  12. dry heat?
    oven: 160-170 C for 2-3 hrs
  13. what is dry heat used for?
    glassware, metal instruments, powers, oils

    flame for loops, needles and lips of tubes
  14. Filtration?
    .2 um pore size used to trap bacteria, .01 um pore size used to trap viruses, vacuum used to speed up process
  15. HEPA filtration?
    high efficiency particulate air filters, removes M.O.'s greater than .3 um from air, used in hospital rooms and laminar flow hoods in laboratories
  16. Thymine Dimers?
    When light hits it, the T's fuse together and create kink in DNA strand. 
  17. actions of chemical agents?
    alter cell wall, altar plasma membrane, damage to proteins and nucleic acids