BacteriologyLabFinalTests

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victimsofadown
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187315
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BacteriologyLabFinalTests
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2012-12-05 14:39:44
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BacteriologyLabFinal
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BacteriologyLabFinal
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  1. Gram stain [results, meaning, next test]
    • Cells stained purple: cell is gram (+) - has thick peptidoglycan layer
    • NEXT - microscopic morphology
    • Cells stained pink: cell is gram (-) - has thin peptidoglycan layer and LPS layer
    • NEXT - microscopic morphology
  2. Microscopic morphology (G+) [results, meaning, next test]
    • coccus:
    • NEXT - catalase test
    • bacillus:
    • NEXT - endospore stain
  3. Microscopic morphology (G-) [results, meaning, next test]
    • coccus:
    • NEXT - none, there are no gram negative cocci
    • bacillus:
    • NEXT - oxidase test
  4. Catalase test [results, meaning, next test]
    • bubbles form: bacteria has catalase (can break down H2O2) [positive]
    • NEXT - glucose fermentation test
    • no bubbles: bacteria can not break down H2O2 [negative]
    • NEXT - blood agar culture
  5. Glucose fermentation test [results, meaning, next test]
    • Tube is yellow (gas or no gas): acid has been produced from anaerobic fermentation [positive]
    • NEXT - acid from mannitol salt agar
    • Tube is red (no gas): acid has not been produced from anaerobic fermentation [negative]
    • NEXT - none, organism is Micrococcus luteus
  6. Acid from mannitol salt agar [results, meaning, next test]
    • Agar has changed from red to yellow: bacteria can ferment mannitol
    • NEXT - lactose fermentation test
    • Agar has remained red: bacteria cannot ferment mannitol
    • NEXT - novobiocin resistance
  7. Lactose fermentation test [results, meaning, next test]
    • Tube is yellow (gas or no gas): acid has been produced from anaerobic fermentation [positive]
    • NEXT - none, organism is Staphylococcus aureus
    • Tube is red (no gas): acid has not been produced from anaerobic fermentation [negative]
    • NEXT - none, organism is Staphylococcus capitis
  8. Novobiocin resistance [results, meaning, next test]
    • area of inhibition greater than 15mm: bacteria is resistance to novobiocin [positive]
    • NEXT - none, organism is Staphylococcus saprophyticus
    • area of inhibition less than 15mm: bacteria is not resistant to novobiocin [negative]
    • NEXT - none, organism is Staphylococcus epidermidis
  9. Blood Agar [results, meaning, next test]
    • greenish color: partial breakdown of RBCs, has alpha hemolysin
    • NEXT - none, no organisms
    • clear area around growth: complete breakdown of RBCs, has beta hemolysin
    • NEXT - none, organism is Streptococcus pyogenes
    • no coloring occurs: no breakdown of RBCs, lacks hemolysin
    • NEXT - Mannitol Salt Agar fermentation
  10. Mannitol salt agar fermentation (after blood agar) [results, meaning, next test]
    • Agar has changed from red to yellow: bacteria can ferment mannitol [positive]
    • NEXT - none, organism is Enterococcus faecalis
    • Agar has remained red: bacteria cannot ferment mannitol [negative]
    • NEXT - none, organims is Streptococcus salivarius
  11. Endospore stain [results, meaning, next test]
    • Green structures can be seen amongst/within red structures: the malachite green has been absorbed by the endospores [positive]
    • NEXT - VP test
    • red structures only can be seen: there are no endospores present [negative]
    • NEXT - acid-fast stain
  12. VP test [results, meaning, next test]
    • MRVP tube turns pink after addition of VP reagant I and II and being exposed to oxygen for 15 minutes: organism produces acetoin from glucose fermentation [positive]
    • NEXT - none, organism is Bacillus subtilis
    • MRVP tube does not turn pink after addition of VP reagant I and II and being exposed to oxygen for 15 minutes: orgaism does not produce acetoin from glucose fermentation [negative]
    • NEXT - none, organism is Bacillus megaterium
  13. Acid-fast stain [results, meaning, next test]
    • Bacteria appears red/pink: did not release carbol-fuchsin during decolorization, IS acid-fast [positive]
    • NEXT - none, organism is Mycobacterium smegmatis
    • Bacteria appears blue: released carbol-fuchsin during decolorization, is not acid-fast [negative]
    • NEXT - none, organism is Lactobacillus rhamnosus
  14. Oxidase test [results, meaning, next test]
    • Oxidase strip turns purple within 50 seconds: has cytochrome C in the ETC [positive]
    • NEXT - oxidized glucose test
    • Oxidase strip does not turn purple within 50 seconds: does not have cytochrome C in th ETC [negative]
    • NEXT - Indole test
  15. Oxidized glucose test [results, meaning, next test]
    • OF glucose medium
    • Exposed tube yellow at top, sealed tube still green: organism is an oxidizer [positive]
    • NEXT - none, organism is Pseudomonas aeruginosa
    • Both tubes completely yellow: organism is a fermenter [negative]
    • NEXT - none, organism is Alcaligenes faecalis
    • Exposed tube slightly blue at top, sealed tube still green: organism does not use glucose [negative]
  16. Indole test [results, meaning, next test]
    • Kovacs reagant turns red: indole production (organism has Tryptophanase) [positive]
    • NEXT - Lactose fermentation with acid AND gas
    • Kovacs reagant remains clear: no Tryptophanase [negative]
    • NEXT - Ornithine decarboxylase (SAME TUBE)
  17. Ornithine decarboxylase (after neg indole) [results, meaning, next test]
    • If MIO tube appears purple: organism has ornithine decarboxylase [positive]
    • NEXT - Urease test
    • If MIO tube does not appear purple: does not have ornithine decarboxylase [negative]
    • NEXT - none, organism is Klebsiella pneumoniae
  18. Lactose fermentation with acid AND gas [results, meaning, next test]
    • Lactose fermentation tube is yellow and has gas: positive
    • NEXT - none, Escherichia coli
    • Lactose fermentation tube is red or yellow w/o gas: negative
    • NEXT - ornithine decarboxylase
  19. Ornithine decarboxylase (after neg lactose ferm w/ gas) [results, meaning, next test]
    • If MIO tube purple: positive
    • NEXT - none, organism is Morganella morganii
    • If MIO tube isn't purple: negative
    • NEXT - none, organims is Proteus vulgaris
  20. Urease test [results, meaning, next test]
    • Urease slant is fuchsia: organism has urease [positive]
    • NEXT - none, organism is Proteus mirabilis
    • Urease slant is yellow: organism does not have urease [negative]
    • NEXT - culture at 25 degrees
  21. Culture at 25 degrees [results, meaning, next test]
    • Organism has red pigment:
    • NEXT - none, organism is Serratia marcescens
    • Organism does not have red pigment:
    • NEXT - none, organism is Enterobacter aerogenes

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