Micro lab final
Card Set Information
Micro lab final
micro lab tests
what is negrosin for?
background dark, cell light. Cell repels negative charge. Morphology.
+ to stain cell itself, sticks to ecll wall. Thick PGW is positive, thin PGW, thick LPS is negative.
nearly pointless unless dealing with bacillus. Add steam to cause panic/formation. Malachite green for spores, saffranin for vegetative cells
endospores in endospore stain
2nd stain in gram, second stain in endospore
glucose, sucrose and lactose with a pH indicater
monosaccharide good for differentiating. Simple.
fermented by enteric organisms, usually
citrate salts as carbon source. utilize difficult environment and break down salts (increase in +ions makes acidic color change
hyrdolyze starch or turn black with iodine. Usually means it's living in the earlier part of the digestive tract
protein (casein) hydrolysis.
lipid hydrolysis (creates lipidase).
chooses one--qualitative. Yes or NO
Selective media for gram +. Broth is better than plate. Kills gram - with alcohol
Selective for gram -, differential for lactose fermentation (green metalic ++, purple +, no color no lactose ferm).
Quantitative. Yes or no, but also how much.
like EMB but contains bile salts. Differential for lactose fermentation (purple) but also selective for gram - and selective for enterics.
mannitol salt agar. Has high levels of salt, so selevtive for facultative halophiles. Differentiates for mannitol (7-C alcohol) fermentation.
: "incomplete". Alters RBCs
: complete destruction of RBCs
: no hemolysis
can it turn H2O2 into O2? Protect itself. Make catalase.