Microbiology week 4

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Author:
torrespeterytania
ID:
190103
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Microbiology week 4
Updated:
2012-12-19 01:32:52
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microbiology week 4
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  1. Culture dependent techniques must be able to grow microbes 
    Give 3 examples
    • Most probable Number 
    • Total heterotrophic plate count 
    • Grow on detection media
  2. Culture-independent techniques test the microbes 
    Give 4 examples 
    • Direct Observation 
    • Chemical 
    • Immunological Methods
    • Nucleic Acid Based 
  3. Define Antigen:
    • Any "thing", foreign to the immune system.e.g.
    • Bacteria, Viruses,(or their parts), Pollen 
  4. Proteins produced by the immune system which help defend against antigens are known as________?
    Antibodies
  5. Antibodies are produced by 
    B cells 
  6. Define Antiglobulins
    Antibodies produced by one species against antibodies of another
  7. Describe Precipitation reactions 
    reaction between soluble antibody + soluble antigen = insoluble product results 
  8. AGGLUTINATION REACTION
    Particulate antigens + antibodies
    • the clumping- together by antibodies, such as RBC or bacteria to form a visible pellet-like precipitate
    • It is a specific reaction (particular antigen clump to specific antibody)
  9. direct agglutination reaction test patient serum vs 
    large cellular antigen to screen for the presence of antibodies 
  10. Indirect agglutination:
    Antibodies vs soluble antigen absorbed to particle or erythrocytes (RBC)
  11. Positive agglutination reaction =
    sufficient antibodies are present in the serum to link antigens together forming a mat of antigen-antibody complexes bottom of well
  12. Non-agglutination=
    not enough antibodies to link antigen

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