MicB 3301-Quiz Material
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Study of Ab/Ag interactions
specific Ag binding with Ab
Immunoprecipitation detects soluble Ag with a visible precipitate
Ouchterlony plate method (aka double diffusion technique)
Uses immunodiffusion to examine the ability of test solutions of Ab and Ag to form stable immune complexes. A ppt line forms on agar between sera and anti-sera that match.
Virulence factors that destruct RBCs and leave alpha or beta hemolysis on BAP
Virulence factors that clot blood plasma and form a protective shield to protect the bacteria from macrophages
Virulence factors that l
yse plasma or fibrin clots; frees organism to spread
Types of exotoxins (lab 16)
- Enterotoxins: stimulate GI cells abnormally
- Cytotoxins: kill host via enzymatic attack
- Neurotoxins: interfere with normal nerve fx
determines the production of diptheria toxin via a known specific Corynebacterium diptheriae antitoxin
Lecithinase and Lecithinase C toxin
method for determining lecithinase production: egg-yolk agar is rich in lecithin. Lecithinase C toxin is produced by Clostridium perfringens. It enables the organism to rapidly spread via edema, necrosis, gas production, and gangrene.
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