Setting Up a Micro Lab
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Describe an ideal micro lab.
- separate room
- good lighting
- good ventilation
- washable floors
- limited traffic
- 2 tables
- smooth surfaces
- gas and electric outlets
- storage space
- access to incubator, autoclave, and refrigerator
What are the materials needed for a microbiology culture?
- gas outlet
- Bunsen burner
- portable propane torch
- gram stain kit
- 3% hydrogen peroxide
- inoculating loops
- sensitivity disks
Which media is used for initial growth and cultivation for further testing?
Columbia Blood Agar
Which media is used to test for antibiotic sensitivity?
What is the gas outlet and Bunsen burner used for?
sterilizing loops and heat fixing slides
What is rabbit plasma used for?
coagulase testing for Staphylococcus aureus
What do we use the autoclave for?
to kill anything that has been cultured in the clinic, including fungal cultures
Can we run materials used for cultures with surgery packs?
no, run them separately
Why do some clinics use old autoclaves for microbiology?
because the bags can leak and it can be very messy
What are the advantages to having an in-house microbiology lab?
- ability to do gram stains
- faster turn around time and more control over the testing process
- less chance of contamination from multiple persons handling samples
- fewer no-growth samples when bacteria are cultured quickly
- less chance of cultures getting lost
What are the disadvantages of having an in-house micro lab?
- takes up tech time
- must have someone who is trained and interested
- unless you are doing micro regularly, supplies can become outdated and need to be replaced
- hospitals are typically laden with bacteria that can contaminate samples
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