MNSs BG System

Card Set Information

Author:
ucstudent01
ID:
195286
Filename:
MNSs BG System
Updated:
2013-01-25 15:02:01
Tags:
MNSs Blood Group System
Folders:

Description:
MNSs BG System
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user ucstudent01 on FreezingBlue Flashcards. What would you like to do?


  1. What 2 genes are responsible for the MNSs System & where is it located?
    Located on Chromosome 4

    • 1st gene is: GYPA - this produces Glycophorin A (GPA).
    • 2nd gene is: GYPB - This produces Glycophorin B (GPB).
  2. What protein structure produces the M and N antigens?
    Glycophorin A
  3. What protein structure produces the S and s antigens?
    Glycophorin B
  4. Remember: Terminology: 3 descriptions

    Haplotype
    Is a linked set of genes inherited together because of their close proximity on a chromosome.

    A set of alleles from closeley linked genes

    A term used in HLA testing to denote the five genes (HLA -A HLA -B, HLA-C, HLA-D,HLA-DR) on the same chromosome.
  5. How do we inherit the MNSs system?
    • From a haplotype
    • aka:
    • a set of alleles from closely linked genes
  6. What does the MNSs haplotype include?
    Both GYPA & GYPB gene
  7. How are closely linked are M and N antigens?
    They are very close. Only on position 1 & 5 on the chromosome do they differ.
  8. If you inherited the M antigen (GPAM) what amino acid is produced at positions 1 & 5?
    • 1: is Serine
    • 5: is Glycine
  9. If you inherited the N antigen (GPAN) what amino acid is produced at positions 1 & 5?
    • 1: is Leucine
    • 5: is Glutamic
  10. If you inherited the S antigen (GPAS) what amino acid is produced at position 29 of the chromosome?
    Methionine will be found
  11. If you inherited the s antigen (GPAs) what amino acid is produced at position 29 of the chromosome?
    Threonine will be present
  12. What are the 4 possible haplotypes (closely linked alleles) for the MNSs system?
    • MS
    • Ms
    • NS
    • Ns
  13. What does Linkage disequilirium mean?
    This is when certain haplotypes are found more frequently than what we expected in the normal population.
  14. What happens if sections of the GPA were deleted?
    • Then the cells would be considered as
    •      En-negative
  15. What happens if sections of the GPB were deleted?
    • Cells would be describes as:
    •           U-negaitve (a heterozygous group)
  16. U-negative RBC's always lack what antigen?
    • Big S
    • &
    • Little s

    so, S-s-
  17. What would the null cell in this blood group sytem be?
    MkNk  (when genes 2-7 of GYPA and genes 1-5 GYPB are deleted)

    These cells will not have MNSs, En or U antignes.

    note: En & U are high incidence
  18. List M & N antigen:
    Significance, antibody class, optimal temp, reaction phase, enzyme treated RBC's enhanced or destroyed
    Is notClinically significant

    Naturally occuring

    IgM

    Agglutination is observed at both 37oC as well as at or below RT (room temp)

    Reacts at: IS, 37C, &  AHG

    Enzyme treatment will destroy postivie agglutinations
  19. List S and s antigen:
    Significance, antibody class, optimal temp, reaction phase, enzyme effects on RBC's
    Clinically Significant

    IgG

    Reacts best at 37oC

    Reaction phases: IS, 37C, or AHG

    Enzymes: ficin and papain inhibit agglutination. Typsin does not destroy reaction.
  20. EN complex is very complicated...So, regarless of the type of EN Cells, if they lack EN...They also lack what other High Incidence antigen?
    Wrb
  21. Wrb is actually part of what blood group and at what location?
    Diego BG System at band 3 protein.
  22. How does Wrb and teh MNSs system interact?
    The GPA and band 3 interact at a cellular level and when GPA is absent form the RBC's, this ineraction can't occur because these cells will not express Wrb.
  23. When might inhance the reaction of Anti- M, N, S, or s?
    Cells that are homozygous rather than heterozygous

What would you like to do?

Home > Flashcards > Print Preview