Card Set Information
Plates and ID
What is MicroStrep used for
and other species - look at the Procedures
How do you setup MicroStep?
Put swab on colonies
Put in Microscan Inoculation Saline - make sure the turbidity is between 0.8-1.2
100 microliters in MicroStrep
Incubate for 20-24 hours
Coag Neg Staph
Beta Lactamase - NEG
Usually won't run on the Instrument and have to do manual
Describe how Proteus can look on plates
Swarming on Chocolate and BAP
Can overpower the CNA plate and grow although it is Gram Negative
Describe E. coli on plates (especially MAC)
Can smell like vinegar
LF or NLF
What specimens/bacterias require contact precautions?
Kleb pneumo (
s - Extended Spectrum Beta-Lactamse, more resistant)
MDRO (like Actinobacter)
Is Pseudomonas Oxidase NEG or POS?
What does Klebsiella look like on a MAC plate?
What antibiotic can differentiate MRSA?
18-20 mm zone is required for sensitivity
What does Staph look like on a plate?
S. aureus - massive beta hemolysis
What does Strep look like on a plate
Very small, translucent colonies
What does Enterococcus look like on a plate
Small translucent colonies
What is used to ID Candida albicans?
Germ tube (2-4 hour incubation time)
(Male Serum in with the species in question)
Positive will show a stem off of the bud
POS - Candida albicans
NEG - Setup Rapid Yeast Panel
What fungus does Firelands report?
What is used to see the spores of fungus?
What is the #1 cause of UTIs?
If a colony is waxy on a Choc plate, colony moves like a hockey puck when pushed, and the specimen is a sputum, What is the most likely species?
(used to be known as Branhamella)
If a colony is sticky on a Chocolate and the species is from a sputum, what is the most likey species?
Is Neisseria Oxidase NEG or POS?
Describe Group B Beta Strep's hemolysis?
The colony usually needs to be moved to see the beta hemolysis
Colony tends to be more translucent
Describe Strep pneumo on a plate
Can have a doughnut look to the colony
Can be mucoid because of the capsule
Describe the D-Test
Klindamycin and Erythromycin discs are used
Need to be 12 mm apart
Used to eval MRSA sensitivity on Klindamycin when the instrument doesn't show sensitivity
How do you eval the D-Test
D zone POS - do not use Clindamycin
D zone NEG - use the Clindamycin
What zone needs to be clear for the P disc and what does it ID?
14 mm clear zone
How do you ID Strep pneumo?
P disc (Optochin)
Oxicillin disc - if POS then the doctors can use this drug
If NEG, setup Microstrep
Do NOT setup Oxicillin if Blood or CSF - automatically setup Microstrep
Instead of Coagulase, what is used to ID Staph aureus?
What resembles Staph in Urines while looking at plates and what test result do they share?
Is Serratia Oxidase Positive or Negative?
Why is the ONPG test used?
To test for delayed lactose fermenters
Where is Providencia found and is it pathogenic or normal flora?
Pathogenic in normal urine
What does Providencia look like on a plate and is it Oxidase Positive or Negative?
What do you do if you are trying to prove if you have a true anaerobe?
Describe the Aerotolerance test
Used to prove if you have a true anaerobe
Sub colonies to a CDC and BAP
CDC goes in the anaerobe bag, while the BAP goes in the aerobic CO
If there is growth on both plates - NEG for anaerobes
If there is growth on just the CDC plate - POS for anaerobes
What is the hemolysis of Group A Strep?
Is Morganella Oxidase Positive or Negative and is it a lactose fermenter?
If you have a Ruptured Appendix, what bacteria would you expect?
If you suspect Gardnerella vaginalis, what plate would you see it on and what test would you do to confirm?
Mostly on Chocolate and CNA (doesn't grow as good on BAP - usually)
Very small, translucent colonies (sometimes has a white center)
Sub to Starch and V agar to confirm (it would grow on both)
Starch - expect blue color
V agar - expect hemolysis
What is the normal pathogenic bacteria expected in Genital Cultures?
What do you sub to for enhanced growth of bacteria in stools?
After 6-8 hours sub the GN Broth to HE to enhance growth of Salmonella and Shigella
What is the distinctive gram stain of Campylobacter?
What are the pathogenic bacteria in the throat?
Strep pneumo (doughnut/coin colony morphology)
What 4 things do you look for to ID Neisseria gonorrhea?
Growth on GC plate
GNDC gram stain
ID on HNID panel
For Strep B cultures, if there is not growth of Strep B on the CNA plate, what do you do automatically?
Sub the LIM broth to BAP
What coded comment is added to Blood Cultures (drawn within the same 24 hrs) if the colonies look like the same species?