Mycology

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Author:
ncrook
ID:
196945
Filename:
Mycology
Updated:
2013-06-01 15:05:47
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Mycology ID
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Description:
Mycology ID
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  1. What organism: 
    Skin scraping
    KOH prep - Spaghetti and meatballs (long hyphae with spherical blastospores)
    Malassezia furfur
  2. Malassezia Furfur
  3. What organism:
    Hard black nodule firmly fixed to the hair shaft
    Dark hypae with ascospores
    Peidraia hortae
  4. Peidraia hortae
  5. What organism:
    Soft white-light brown nodules on the hair shaft that are gelatinous and can crumble
    Dense network of mycelia along hair with arthrospores and blastospores
    Triichosporon beigelii
  6. Trichosporon beigelii
  7. What organism:
    Skin and hair
    Colony morphology:  Top - Velvety light tan-white, Reverse - Salmon on a PDA
    Rare macro and microconidia Raquet hyphae, terminally located usually pointed chlamydospores (thicker walls)
    Microsporum audounii
  8. Microsporum audounii
  9. What organism:
    Skin and hair
    Colony morphology:  Top - White and Wooly (Buff brown center) with yellow orange perimeter, Reverse: Bright yellow orange
    Abundant macroconidia spindle shaped, thick walled (5-14 septa), few clavate microconidia
    Microsporum canis
  10. Microsporum canis
  11. What organism:
    Skin and hair
    Colony Morphology:  Top - matted granular powdery buff-cinnamon brown, Reverse - tan-mahogany red
    Microsporum gypseum
  12. Microsporum gypseum
  13. What organism:
    Microscopic morphology:  Downy type - rare macroconidia, numerous to few microconidia in grape-like clusters; Granular type - spiral hyphae, pencil shaped, thin walled macroconidia with 5-8 cells, grape-like microconidia
    Urease POS in 2-4 days
    Hair-baiting POS in 10-14 days
    PDA - NO PIGMENT
    Trichophyton mentagraphytes
  14. Trichophyton mentagraphytes (Downy)
  15. What organism:
    Colony morphology:  Top - granular or fluffy white with pink perimeter; Reverse - Deep cherry red (PDA 2 weeks)
    Urease NEG
    Hair-baiting NEG
    Trichophyton rubrum
  16. Trichophyton rubum
  17. What organism:
    Urease POS after 4 days
    Hair-baiting - NEG
    Trichophyton tonsurans
  18. Trichophyton tonsurans
  19. What organism:
    Microscopic morphology: Rare macroconidia and microconidia, very vegetative structures form favic chandeliers (like moose antlers)
    Trichophyton schoenleinii
  20. Trichophyton schoenleinii
  21. What organism:
    Colony morphology: Dimorphic fungi, use BHI w/10% SB to convert from mold to yeast; Yeast - soft, white/cream colored; Mold - Moist, pink/cream colored, becomes wrinkled leathery, and black with age
    Microscopic morphology: Yeast - cigar shaped budding yeast (fusiform); Mold - flower-like (daisy head) pyriform conidia at the tip of the short conidophores
    Sporothrix schenckii
  22. Sporothrix schenckii (Yeast)
  23. What organism:
    Aspirate or drainage from active lesions histology or direct smear shows sclerotic bodies (copper pennies) - diagnostic for chromomycosis
    Colony morphology: Top - dark brown/black velvet (dematiaceous) colonies; Reverse - Black with small folds
    Microscopic morphology: Cladosporia
    Cladosporum carionii
  24. Cladosporium carionii
  25. Pseudallesheria Boydii (sexual name) or Scedosporium apiospermum (asexual name)
  26. What organism:
    Aspirate or drainage from active lesions, histology or direct smear shows sclerotic bodies (copper pennies) - diagnostic for chromomycosis
    Colony morphology: Top - dark brown/black cottony, small mound (dematiaceous); Reverse - black (no folds)
    Microscopic morphology: Rhino-cladiella, cladosporia, phialophora
    Fonsecaea pedrosoi
  27. Fonsecaea pedrosoi
  28. What organism:
    Aspirate or drainage from active lesions, histology or direct smear shows sclerotic bodies (copper pennies) - diagnostic for chromomycosis
    Colony morphology: Dark brown/black (dematiaceous)
    Microscopic morphology: Rhino-cladiella, cladosporia, phialophora
    Fonsecaea compactum
  29. Fonsecaea compactum
  30. What organism:
    Aspirated fluid or drainage direct smear will  show sulfur granules in drainage (yellow or white 0-2 mm irregular shaped)Colony morphology: cottony, mousy gray in color
    Microscopic morphology: Sexual growth - cleistothecium with ascospores; Asexual growth - "lollipop" conidia or balloons on a string
    • Pseudallesheria boydii (sexual name) 
    • Scedosporium apiospermum
  31. What organism:
    Direct exam: India ink - budding yeast cells with capsules
    Gram stain Positve or Variable due to capsule
    Urease POS
    TOC agar - brown pigmented colonies (pigment formed by the reaction of phenol oxidase with caffeic acid in media)
    Nitrate NEG
    Best ID: Serological Cryptococcal latex antigen test
    Crytococcus neoformans
  32. Cryptococcus neoformans
  33. What organism:
    30 Degree Celcius: Arthrospore with every other cell an empty disjunctor cell - highly contagious
    37 Degree Celcius: Large spherule (like sporangia) filled with endospores
    Coccidiodes immitis
  34. Coccidiodes immitis
  35. What organism:
    30 Degree Celcius:  Tuberculated macroconidia
    37 Degrees Celcius: Budding yeast cells intracellular in Res
    Histoplasma capsulatum
  36. Histoplasma capsulatum
  37. What organsim:
    30 Degrees Celcius: Round conidia at ends of short condiophores
    37 Degrees Celcius: Thick walled single broadbased budding yeast cells
    Blastomyces dermititidis
  38. Blastomyces dermititidis
  39. What organism:
    30°C: NUMEROUS CHLAMYDOCONIDIA                
    37°C:  THICK WALLED MULTIPLE BUDDING YEAST CELLS "MARINERS WHEEL"
    PARACOCCIDIOIDES BRASILIENSIS
  40. PARACOCCIDIOIDES BRASILIENSIS
  41. What organism:
    INFECTS AND INVADES ARTERIAL VESSELS RESULTING IN EXTENSIVE THROMBOSIS (RARE BUT OFTEN FATAL WHEN THROMBOSIS IN BRAIN CAUSES NECROSIS AND DEATH)
    Colony Morphology: TOP:  WHITE COTTONY WITH BLACK SPORES TOUCHING TOP OF LID (OILY WHEN OLDER)
    Microscopic morphology: SPORANGIPHORE RISE NODALLY FROM THE RHIZOIDS and ASEPTATE HYPHAE W/ SPORANGIUM
    RHIZOPUS
  42. RHIZOPUS

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