Clinical Microbiology Chapter 14

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cruz_852000
ID:
198240
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Clinical Microbiology Chapter 14
Updated:
2013-02-07 18:53:49
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Staphylococci
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Description:
chapter 14 staphylococci (clinical micro)
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  1. Characteristics of Staphylococci
    • -Gram positive cocci
    • -Catalase Positive
    • -Facultative anaerobes
    • -Non-motile
    • -Non-spore forming
  2. Staphylocccous aureus
    • -Gram Positive cocci (clusters and singly)
    • -Beta Hemolytic colonies (BA and CNA agar)
    • -Catalase +
    • -Coagulase +
  3. Infections associated with Staphyloccoccus aureus
    • -Skin and wound infections 
    • -Pneumonia
    • -Osteomyelitis and Septic Arthritis
    • -Food Poisoning
    • -UTIs
    • -Toxic Shock Syndrome
    • -Scalded Skin Syndrome
  4. MRSA
    • (Methicillin Resistant Staphylococcus aureus)
    • -Approximately 50% of Staph aureus isolates are MRSA
    • -Sensitive to Vancomycin
  5. Staphylococcus epidermidis
    • -Gram positive cocci (clusters and singly)
    • -Non-hemolytic colonies (BA and CNA agars)
    • -Catalase +
    • -Coagulase =
    • -Novbiocin Sensitive (S)
  6. Infectious conditions associated with Staphylococcus epidermidis
    • -Normal flora (skin)
    • -Contaminant
    • -Opportunistic pathogen
    • -Nosocomial UTI
    • -Heart Valve infections
    • -Catheters and shunts (IV lines)
  7. Staphylococcus saprophyticus
    • -Gram positive cocci
    • -Non-hemolytic (BA and CNA agar)
    • -Catalase +
    • -Coaguase =
    • -Novobiocin Resistant (R)
    • -Common cause of UTI in young women
    • -Generally susceptibility to antibiotics
  8. Micrococci
    • -Gram positive cocci in tetrads 
    • -Non-hemolytic
    • -Catalase +
    • -Coagulase =
    • -Bacitracin Sensitive (S)
    • -Occasional pathogen
  9. Catalase test
    Catalase is a bacterial enzyme that breaks down hydrogen peroxide

     
  10. Coagulase Test
    Detects clumping factor (bound coagulase attached to the bacteria)
  11. Novobiocin Resistance Test
    • -Antibiotic susceptibility test used to differentiate Staph epidermidis from Staph saprophyticus
    • -C-disk 
    • -Susceptible > 16mm
    • -Resistant < 16 mm
  12. Sheep Blood Agar
    (BA)
    Demonstrates hemolytic patterns
  13. Mannitol Salt Agar
    (MSA)
    • inhibits non-Staphylococcus
    • -Mannitol fermenters (Staph aureus) are yellow
    • -Non-fermenters pink
  14. Chrome Agar MRSA
    • -Screening media for MRSA from nasal swabs
    • -MRSA mauvre, other organisms inhibited
  15. Oxacillin Screen agar
    Differentiates MRSA from non-MRSA

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