Lecture 5

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Lecture 5
2013-02-07 13:07:47
BIS 104

ER protein entrance, transport type, evolution scheme
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  1. Secondary active transport
    Energy stored in ion gradient can drive transport systems. Does not utilize ATP.
  2. Uniport
    One molecule moving across membrane.
  3. Symport
    One molecule and one ion moving across the membrane.
  4. Antiport
    One molecule and one ion moving in different directions across membrane.
  5. Hypothetical scheme fro evolution of nucleus
    • 1. DNA bound to inner membrane.
    • 2. Membrane folds in and¬†encompasses DNA.
    • 3. A double membrane is formed.
    • ¬†
  6. Hypothetical scheme for origin of mitochondria and plastids
    • 1. Aerobic prokaryotic cell comes close to eukaryotic cell.
    • 2. Membrane engulfs prokaryotic cell.
    • 3. Mitochondria has two membranes now.
  7. Cytosol is a ______ environment.
    Outside of cell is an ______ environment.
    reducing, oxidizing
  8. Blobel's in vitro translocation
    1. Tubes with radiolabelled amino acids, mRNA encoding secretory protein, and one with and without microsomes.

    2. Added protease to each tube and saw that the one without microsome had its protein destroyed.
  9. Signal for transport/import of proteins into ER
    10-20 hydrophobic amino acids
  10. Signal Recognition Particle
    Binds to working ribosome and connects it to ER membrane. Once ribosome has been connected it SRP gets displaced.
  11. Translocator
    Protein on ER membrane. 3-4 protein complex acting as pore for ribosome protein entrance.