Microbiology Media

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cruz_852000
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198762
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Microbiology Media
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2013-02-10 22:29:27
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Microbiology Media
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Media
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  1. Sheep Blood Agar
    (BA)
    • Primary Use: Enriched media
    • Nutrients: Trypticase soy agar with 5% sheep blood
    • Indicators: Sheep RBCs may demonstrate alpha, beta or gamma hemolysis.
    • Comments: Supports most fastidious and non-fastidious organisms. Incubate at increased CO2
  2. CNA agar
    (CNA)
    • Primary Use: Selective media to recover gram positive bacteria
    • Nutrients: Trypticase soy agar with 5% sheep blood
    • Inhibitors: Colistin, Nalidixic acid, Gram neg inhibited
    • Comments: Inhibits growth of gram-negative bacteria from mixed cultures. Incubate at increased CO2
  3. MacConkey Agar
    (MAC)
    • Primary Use: Selective-Differential media to isolate gram negative bacteria. Differentiates lactose and non-lactose fermenters.
    • Nutrients: Carbohydrate- Lactose
    • Inhibitors: Bile salts, Neutral red, Crystal violet 
    • Indicators: ph indicator - neutral red
    • Comments: Lactose is only carbohydrate
    • Lactose Fermentors: pink-red
    • Non-Lactose Fermentors: colorless
    • Incubate in aerobic incubator
  4. Chocolate agar
    (CHOC)
    • Primary Use: Non-selective enriched media. Supports the growth of most fastidious bacteria
    • Nutrients: Trypticase soy agar, hemolyzed sheep blood, IsoVitaleX
    • Commentes: Supports most fastidious organisms such as N. gonorrhoeae & Haemophilus lysed RBCs released heme (X Factor) & Incubate at increased CO2
  5. Thioglycolate Broth
    (THIO)
    • Primary Use: Enriched broth used to support growth of most bacteria, including anaerobes and microaeroophils. Routienely used for wound and sterile body fluid cultures
    • Nutrients: Casein, Soy broth, Glucose
    • Inhibitors: Thioglycolate, cystine and sodium sulfite reduce oxygen.
    • Comments: Boil before use to expel dissolved OObligative anaerobes grow only at bottom.
  6. Modified Thayer - Martin Agar
    (MTM)
    • Primary Use: Selective media to isolate Neisseria gonorrhoeae & Neisseria meningitidis. Always included with genital cultures or from any source when looking for N. gonorrhoeae
    • Nutrients: Chocolate agar base
    • Inhibitors: Antibiotics - Colistin (Gram =) Nystatin (yeasts) Vancomycin (Gram +) Trimethoprim (Proteus)
    • Comments: Antibiotics inhibit growth of normal flora from mixed cultures. Incubate at increased CO2
  7. Related media to Modified Thayer - Martin Agar (MTM)
    • Martin-Lewis
    • GC Lect
    • New York City: not blood based
  8. Oxacillin Screen Agar
    • Primary Use: Test for MRSA (Methicillin Resistant Staph aureus)
    • Nutrients:Mueller Hinton agar with ocacillin
    • Inhibitors: Oxacillin (6 um/ml)
    • Comments: MRSA is resistant and grows, Non-MRSA is inhibited
  9. Chrom Agar MRSA
    • Primary Use: Selective-Differential agar for MRSA screening (nasal swabs). MRSA screening only - NOT a definitive ID
    • Inhibitors: MRSA strains will grow in the presence of cefoxitin. Other organisms inhibited.
    • Indicators: MRSA hydrolysis of chromogen
    • Comments: Incubate aerobically for 24 hrs. MRSA = Mauve colored colonies. Other Staph= white colonies. Incubate 48 hrs. if no colonies @ 24 hrs.
  10. Vancomycin Screen Agar
    • Primary Use: Test for VRE (Vancomycin Resistant Enterococci) Used to test Enterococcus isolates only
    • Nutrients: BHI, Peptones
    • Inhibitors: Vancomycin (6 um/ml)
    • Comments: Incubate aerobically 24hrs at 35C Growth indicates that the isolate is VRE and resistant to VRE
  11. Streptococcal Selective agar (SSA)
    • Primary Use: Selective media to isolate Strep. pyogenes and Strep agalactiae
    • Nutrients: Sheep blood agar base
    • Inhibitors: Crystal violet, Colistin, Trimethroprime, Sulfamethoxazole
    • Comments: Normal respiratory flora inhibited. Selective for Group A and Group B Strep. Incubated anaerobically
  12. Todd - Hewitt Broth
    • Primary Use: Selective broth used to culture GBS from vaginal / rectal swabs
    • Nutrients: Peptones, Yeast extract
    • Inhibitors: Nalidixic acid, Gentamycin
    • Comments: Vaginal or rectal swabs collected at 35-37 weeks gestation, incubated @ 35C for 24 hrs, then subculture to blood agar. Blood agar examined at 24 and 48 hrs.
  13. LIM Broth
    • Primary Use: Selective-Enrichment broth to isolate Group B Strep.
    • Nutrients: Peptones, Yeast extract
    • Inhibitors: Nalidixic acid, Colistin
    • Comments: Included with female genital cultures. Largely replaced by Todd - Hewitt Broth
  14. Mannitol Salt Agar (MSA)
    • Primary Use: Selective-Differential media to isolate Stap. species
    • Nutrients: Peptone base, Mannitol
    • Inhibitors: 7.5% NaCl
    • Indicators: pH indicator - Phenol Red
    • Comments: High salt concentration inhibits most bacteria except Staph. Mannitol fermenters - yellow colonies
  15. Phenylethyl Alcohol Agar (PEA)
    • Primary Use: Selective media to recover gram positive bacteria. May be used as an alternative to CNA agar
    • Nutrients: Nutrient agar, 5% Sheep Blood
    • Inhibitors: Phenylethanol inhibits the growth of gram negative organisms.
    • Comments: Inhibits growth of gram-negative bacteria from mixed cultures. Incubate at increased CO2
  16. Mueller - Hinton Agar (MH)
    • Primary Use: Enrichment media routinely used to perform antibiotic susceptibility testing.
    • Nutrients: Beef extract, Casein, Starch
    • Comments: Sheep RBCs may be added to support the growth of Strep and other fastidious organisms. 
    • Do NOT incubate in CO2 incubator
  17. Sabouraud Dextrose agar
    • Primary Use: Primary media for the isolation of fungi
    • Nutrients: Peptones, Glucose
    • Inhibitors: Low pH (5.6) inhibits bacteria
    • Comments: Antibotics- Chloramphenicol and gentamycin can be added to inhibit bacterial growth
  18. Tinsdale agar
    • Primary use: Selective-Differential agar for the isolation of Corynebacterum diptheriae
    • Inhibitors: Potassium tellurite inhibits respiratory normal flora
    • Indicators: Tellurite
    • Comments: C. diphtheria colonies are metallic black with a brown halo.
  19. Tinsdale agar related media
    • Cystine-Telurite agar
    • Loeffler's media
  20. Haemophilus ID Quad Plate
    • Primary Use: Enriched media with the selective addition of X, V, XV and Horse RBCs to each of the 4 divided quadrants. Used to ID Haemophilus species
    • Comments: 
    • 1st Quad = X Factor
    • 2nd Quad = V Factor
    • 3rd Quad = X and V Factor
    • 4th Quad = X and V, Horse RBCs
  21. Haemophilus Test Medium (HTM)
    • Primary Use: Enriched media for disk antibiotic susceptibility testing on Haemophilus.
    • Nutrients: Beef extract, Yeast extract, NAD, Hematin
    • Comments: Although susceptibility testing is not routine for Haemophilus, HTM is used for beta-lactamase positive isolates.
    • Incubate in CO2 incubator
  22. Vaginalis Agar (V Agar)
    • Primary Use: Selective media to isolate Gardnerella species from cultures
    • Nutrients: Human blood, Starch
    • Inhibitors: Colistin, Nalidixic acid, Nystain
    • Comments: Genital normal flora inhibited. Gardnerella colonies are small, dome-shaped beta-hemolytic colonies
  23. HBT Bilayer Agar
    (Human Blood Tween 80)
    • Primary Use: Selective media to isolate Gardnerella species from genital cultures
    • Nutrients: Human blood, Tween 80
    • Inhibitors: Colistin, Nalidixic acid, Amphotericin B
    • Comments: A thin layer of 5% human blood overlays CNA agar. Gardnerella is beta-hemolytic on human blood
  24. Bordet-Genou Agar
    • Primary Use: Selective-Differential media to isolate Bordatella pertussis.
    • Nutrients: Potato-glycerol enriched with 15-20% blood
    • Inhibitors: Methicillin
    • Comments: For isolation of Bordatella pertussis-colonies have "mercury" appearance
  25. Bordet-Genou Agar related media
    Regan-Lowe media
  26. Brain Heart Infusion Broth (BHI)
    • Primary Use: Enriched media used for the growth of fastidous organisms
    • Nutrients: Beef heart extract, Tryptose
  27. Blood Culture Bottle Broth
    • Primary Use: Enriched broth to grow just about everything
    • Nutrients: Soy-Casien, Yeast extract, Carbohydrates, X and V factors
    • Indicators: CO2 production by living organisms creates fluorescent light from disk indicator
    • Comments: SPS anticoagulant/Anti-Complement Antibiotic absorbing resins
  28. BCYE media (Buffered Charcoal Yeast Extract)
    • Primary Use: Enriched media for the isolation of Legionella species.
    • Nutrients: Yeast extract, Cysteine, Iron salts, alpha-ketoglutarate
    • Inhibitors: Vancomycin, Colistin, Antifungals, Charcoal absorbs toxic substances
    • Comments: Isolation of Legionella, but also supports growth of other fastidious organisms-Bordatella, Brucella and Francisella
  29. Lysozyme Broth
    • Primary Use: Selective broth used to differentiate Nocardia from Streptomycin species
    • Nutrients: Beef extract, Casein, Glycerol
    • Inhibitors: Lysozyme (enzyme)
    • Comments: Nocardia resistan to Lysozyme (growth). Streptomyces sensitive to lysozyme (no growth)
  30. Hektoen Enteric Agar (HE)
    • Primary Use: Selective-Differential media to isolate Salmonella and Shigella. Always included for stool cultures
    • Nutrients: Carbohydrates- Lactose, Sucrose, Salicin
    • Inhibitors: Biles salts
    • Indicators: pH indicators: Brom-thymol blue and acid ffuchsin. H2S production: sodium thiosulfate and ferric ammonium citrate
    • Comments: Gram pos. and normal flora inhibited. Fermenters: Orange-yellow
    • Salmonella: blue-green, black center
    • Shigella: green fading to periphery
  31. MacConkey with Sorbitol Agar
    (Mac-Sorb)
    • Primary Use: Selective-Differential media to isolate E. coli O157:H7
    • Nutrients: Carbohydrate: Sorbitol
    • Inhibitors: Bile Salt, Neutral red, Crystal violet
    • Indicators: pH indicator: neutral red
    • Comments: Lactose is replaced with sorbitol. Used to set up bloody stools. E. coli O157 forms colorless colonies. Normal E. coli ferments sorbitol
  32. Sorbitol-MacConkey II with Cefixime and Tellurite (MAC-Sorb-CT)
    • Primary Use: Selective-Differential media to isolate and presumptively ID E. coli O157:H7
    • Nutrients: Carbohydrate: Sorbitol
    • Inhibitors: Cefixime and Tellurite, Bile salts, Neutral red, Crystal violet
    • Indicators: pH indicator: neutral red
    • Comments: Cefixime and Tellurite inhibit Proteus mirabilis, non E. coli O157:H7 and other non-srbitol fermenters
  33. Salmonella-Shigella Agar (SS)
    • Primary Use: Selective-Differential media to isolate Salmonella and Shigella
    • Nutrients: Carbohydrate: Lactose
    • Inhibitors: Bile salts, Sodium citrate
    • Indicators: pH indicator: neutral red. H2S production: sodium thiosulfate and ferric citrate
    • Comments: Gram positive and coliforms inhibited. Lactose only sugar. LF: red. NLF: colorless. H2S production: black center
  34. Xylose-lysine deoxycholate Agar (XLD)
    • Primary Use: Selective-Differential media to isolate Shigella
    • Nutrients: Carbohydrates: Lactose, Sucrose, Xylose
    • Inhibitors: Bile salts
    • Indicators: pH indicator: phenol red H2S production: sodium thiosulfate and ferric ammonium citrate
    • Comments: Salmonella: red/black centers. Shigella, providence: Clear. LF: Yellow
  35. Eosin-Methylene-Blue Agar (EMB)
    • Primary Use: Selective-Differential media to isolate gram negative bacteria. May be used as an alternative to MacConkey agar. 
    • Nutrients: Carbohydrate: Lactose
    • Inhibitors: Eosin, Methylene blue
    • Indicators: Eosin, Methylene blue
    • Comments: Gram positive bacteria inhibited. Lactose is only carbohydrate. 
    • LF: green-black, green metallic sheen
    • NLF: clear
  36. Yersinia agar (CIN agar)
    • Primary Use: Selective-Differential media for the isolation of Yersinia enterocolitica.
    • Nutrients: Carbohydrate: Mannitol
    • Inhibitors: Cefsulodin, Irgasan, Novobiocin, Bile salts, Crystal violet
    • Comments: "Bulls-eye" colonies. May also be use to isolate Aeromonas from stool specimens
  37. GN Broth (Gram Negative)
    • Primary Use: Selective-Enrichment broth used to isolate enteric pathogens.
    • Nutrients: Peptones, Mannitol, Glucose
    • Inhibitors: Sodium Citrate and sodium deoxycholate inhibit most non-enteric pathogens and gram positives. 
    • Comments: Routinely used for stool cultures. Subculture to HE after 4 hrs incubation.
    • Not in common use
  38. Cepacia Selective Agar (BSCA)
    • Primary Use: Selective/Differential agar for isolation of Burkholderia cepacia in Cystic Fibrosis patients
    • Nutrients: Yeast extract, Peptones, sucrose, lactose
    • Inhibitors: Polymixin B, gentamycin, vancomycin, crystal violet
    • Comments: B. cepacia colonies are rough to smooth purple colonies with yellow color around colony
  39. TCBS agar (Thiosulfate-Citrate-Bile salts-Sucrose)
    • Primary Use: Selective-Differential media for the isolation of Vibrio species
    • Nutrients: Carbohydrate: Sucrose
    • Inhibitors: Most gram-positives and gram-negatives inhibited
    • Indicators: Bromthymol blue, H2S
    • Comments: Sucrose fermenters: Yellow (Vibrio cholera)
    • Sucrose non-fermenters: Blue-Green
  40. Campylobacter Agar (CVA)
    • Primary Use: Selective media to isolate Camplobacter species from stool cultures.
    • Nutrients: Brucella agar base with RBCs
    • Inhibitors: Cefoperazone, Vancomycin, Amphotericin
    • Commentes: Routienly used for stool cultures. Incubated at 42C
    • GI normal flora inhibited
  41. Campylobacter Agar with 10% Blood (Campy)
    • Primary Use: Selective media to isolate Campylobacter species from stool cultures.
    • Nutrients: Brucella agar base with RBCs
    • Inhibitors: Vancomycin, Trimethoprim, Polymixin B, Amphotericin B, Cephalothin
    • Comments: Routinely used to set up stool cultures. Incubate at 42C
    • GI normal flora inhibited
  42. Anaerobic Blood Agar (CDC ANA)
    • Primary Use: Enriched media for the isolation of obligate gram negative and gram positive anaerobes.
    • Nutrients: 5% sheep blood, Vitamin K, Vitamin B, Yeast extract
    • Comments: Incubated anaerobically.
  43. Kanamycin-Vancomycin Laked Blood Agar (KVLB)
    • Primary Use: Selective isolateion of anaerobes Bacteroides and Prevotella
    • Nutrients: 5% sheep blood
    • Inhibitors: Kanamycin, Vancomycin
    • Comments: Kanamycin inhibits most facultative gram negative rods (enterics) Vancomycin inhibits most gram postitive "Laked" = hemolyzed 
    • Incubated anaerobically
  44. Anaerobic PEA (ANA PEA)
    • Primary Use: Slective media for isolation of obligate anaerobes
    • Nutrients: 5% sheep blood
    • Inhibitors: Phenylethanol inhibits most facultative gram negative rods.
    • Comments: Incubate anaerobically
  45. Bacteroides Bile-Esculin Agar (BBE)
    • Primary Use: Selective-Differential agar to isolate and presumptively ID Bacteroides fragilis
    • Inhibitors: Gentamycin, Bile salts
    • Inicators: Esculin hydrolyzed to esculetin (black)
    • Comments: Bacteroides fragillis colonies form black-brown colonies
  46. Cooked Meat Broth
    • Primary Use: Enriched broth useful for the growth of obligative anaerobes
    • Nutrients: Glucose, Heme, Vitamin K, Yeast extract, Tissue proteins
    • Comments: Supports growth of fastidious obligative anaerobes, especially clostridium
  47. Brucella Media for anaerobes
    • Primary Use: Brucella Agar with 5% Sheep Blood, Hemin and Vitamin K1 is used for the isolation and cultivation of fastidious, obligately anaerobic microorganisms
    • Nutrients: Heme, Vitamin K, Peptones, Dextrose and yeast extract.
    • Inhibitors: Kanamycin and vancomycin are included in Brucella Laked Blood KV Agar for use in selective isolation of gram-negative anaerobes, expecially Bacteroides.
  48. Lowenstein-Jensen
    • Primary Use: Non-selective media for the isolation of Mycobacteria
    • Nutrients: Eggs, potato flour, glycerol
    • Inhibitors: Malachite green
  49. Middlebrook
    • Primary Use: Non-selective media for the isolation of Mycobacteria
    • Nutrients: Oleic acid, Albumin
    • Inhibitors: Malachite green
  50. Casein/Tyrosine/Xanthine agars
    • Primary Use: Differential media used to differentiate Noacardia and Streptomyces species
    • Nutrients: Beef extract
    • Comments: Incubate agar 48-72 hrs. Add Gram's iodine to the surface. Hydrolysis=Clear zone around/under colony
    • No hydrolysis = No clearing

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