Microbiology ID test

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Author:
cruz_852000
ID:
199695
Filename:
Microbiology ID test
Updated:
2013-02-11 11:09:42
Tags:
microbiology
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Description:
Microbiology ID test
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  1. Catalase test
    • Catalase is a bacterial enzyme that breaks down hydrogen peroxide
    • Positive = Bubbling
    • Negative = No Bubbling
    • Differentiate Staph and Strep
  2. Slide Coagulase Test
    • Detects Clumping Factor (bound coagulase attached to the bacteria)
    • Positive = Visible clumping of latex
    • Negative = No clumping
  3. Tube Coagulase Test
    • Coagulase is a bacterial enzyme that clots plasma
    • Inoculate bacterial paste to rabbit plasma
    • Incubate 4 hrs at 35C
    • Positive = clotting
    • Negative = No clotting
    • A back-up or confirmation test to the faster slide coagulase
  4. Novobiocin Resistance Test
    • Antibiotic susceptibility test used to differentiate Staphylococcus epidermis from Staphylococcus saprophyticus
    • -"Carpet streak" BA w/ inoculum
    • -Place C-disk on agar surface
    • -Incubate 24hrs at 35C
    • Susceptible > 16mm
    • Resistant < 16mm

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