Anaerobic bacteria I

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Anaerobic bacteria I
2013-02-12 12:53:13
micro exam

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  1. obligate aerobes
    Absolute requirement for O2 togrow
  2. ¢Obligate anaerobes
    • —Grow only under conditions of high reducing intensity
    • —O2  is toxic
    • growth only in anaerobic system with 0% O2
  3. ¢Facultative anaerobes
    —Grow under aerobic or anaerobic conditions
  4. ¢Microaerophiles
    • —Grow best under lower O2 intensity
    • —Higher O2 tensions may be inhibitory
    • growth in anaerobic system 0% O2 and in 10% CO2, 85% N2, 5% O2
    • No growth in CO2 incubator w/ 5% CO2, 15% O2 also no growth in ambient air w/ about 21% O2
  5. ¢Aerotolerant anaerobes
    —Anaerobes that are not killed by exposure to O2
  6. anaerobic, spore-forming, gram pos, bacilli
    • Clostridium
    • ¢C. perfringens, C. difficile, C. botulinum, C. histolyticum, C. septicum, C. tetani
  7. anaerobic, non-spore forming, gram pos, bacilli (6)
    • ¢Actinomyces spp
    • ¢Bifidobacterium spp
    • ¢Eubacterium spp
    • ¢Lactobacillus spp
    • ¢Mobiluncus spp
    • ¢Propionibacterium spp
  8. anaerobic, gram pos, cocci, non-spore forming
    • ¢Peptostreptococcus spp
    •       ¢P. anaerobius
    •       ¢P. asaccharolyticus
    • —Staphylococcus saccharolyticus—
    • Streptococcus spp
  9. anaerobic, gram neg bacilli
    • —Bacteroides
    • —Bilophila
    • —Campylobacter—
    • Fusobacterium
    •       ¢F. mortiferum
    •       ¢F. necrophorum
    •       ¢F. nucleatum
    • —Porphyromonas
    •       ¢P. asaccharolytica
    • —Prevotella
    •       ¢P. intermedia
    •       ¢P. melaninogenica
  10. anaerobic, gram neg cocci
    • —Veillonella  —
    • Megasphaera spp.
  11. Strict anaerobes can die within _____ minutes of O2 exposure
    10 minutes
  12. Name 3 enzymes that moderate anaerobes can use to protect them against toxic effects of O2
    • ¢Most moderate anaerobes survive much longer
    • —Produce enzymes like catalase, superoxide dismutase and peroxidase that protect them against toxic effects of O2 production—Direct correlation between O2 tolerance of an organism and its virulence
    • —Some organisms do not produce these enzymes but still have a high level of virulence