PHYSIO LAB MIDTERM QUESTIONS. SLCC

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PHYSIO LAB MIDTERM QUESTIONS. SLCC
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2013-02-27 19:45:34
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PHYSIO LAB MIDTERM QUESTIONS SLCC
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PHYSIO LAB MIDTERM QUESTIONS. SLCC
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  1. T or F: Homeostasis is the ability of the body or a cell to seek and maintain an condition of stability within its internal environment when dealing with the external changes?
    True
  2. Which of the following would be an example of a hypothesis?
    D. Man-made pollution causes global warming.
  3. When graphing data, the dependent variable is on the ___ axis and the independent variable is on the ___ axis?
    Y,X
  4. Circadian rhythms are ___ rhythmic changes in behavior or physiological processes?
    Daily
  5. What is the median of the following set of numbers: 2,10,20,25,60,75,101?
    25 (what # is in the middle?)
  6. What is the mode of the following set of numbers: 10,5,12,8,6,5,21,15? 
    5 (the # that appears the most)
  7. What is the range of the following set of numbers? 10,50,100,200,500?
    490 (lowest # subtracted from the highest #)
  8. What is the standard deviation of the follow set of numbers: 1,3,6,10?
    • 3.9
    • 1. Find the mean of each #
    • 2. Subtract the mean from each #
    • 3. Square each answer (multiply each by itself)
    • 4. Add each answer together.
    • 5. Divide by the number of #’s -1
    • 6. Square root the answer
  9. _____ refers to how close to the actual or true value of a measurement is?

    FILL IN THE BLANK
    Accuracy
  10. _____ refers to the repeatability of the measurement?

    FILL IN THE BLANK
    Precision
  11. What is the BMI of a male who weighs 77.8kg and is 1.8 meters?
    24 (w/h2)
  12. Biruet Reagent (coomassie blue) reacts with which of the following substances to determine its concentration in a solution?
    Proteins
  13. If you want to make a 05. Liter of a 1 M solution of NaCl using a 4 M stock solution of NaCl, how much of the stock solution would you have to add?
    125 ml (c1v1=c2v2)
  14. The greater the number of particles in a solution, the ____ the Optical Density (OD)? 
    Higher
  15. Given the data in the following table, find the unknown (BSA) ug/ml in cuvette6.  Use the x-y graph at right to plot both the standard curve and the unknown BSA concentration
    750
  16. T or F Diffusion is a physical process in which molecules move from an area where there are in high concentration to one where their concentration is lower
    True
  17. Which of the following factors do not affect diffusion?
    pH
  18. T or F Permeable solute affects tonicity across a semi-permeable membrane? 
    False
  19. If you took a red blood cell (RBC) and placed it in a beaker with water from the Great Salt Lake, what would occur?
    The red blood cell would crenate
  20. If a RBC is placed in a hypotonic solution then water will___?
    Flow into the cell
  21. If a RBC is placed in a hypertonic solution, then water will ___?
    Flow out of the cell
  22. T or F If nothing hinders the movement of a solute, it will diffuse until it reaches equilibrium. 
    True
  23. What is the osmolarity of a 20% glucose solution? (Hint: the MW of glucose is 180g/mole and 20% solution contains 20g/100ml.) Show your work
    mole/L = 20g/100ml * mole/180g* 1000ml/1L* 1= ___osmoles/L
  24. What is the osmolarity of a 10% MgCl2 solution? (hint: the MW of MgCl2 is 95g/mole and a 10% solution contains 10g/100ml.) Show your work
    mole/L = 10g/100ml * mole/95g* 1000ml/1L* 2= ___osmoles/L
  25. uppose you had solutions in questions 23 and 24 separated by a membrane in a beaker.  If only water could pass through the membrane, which way would it diffuse?
    Toward the MgCl2
  26. Which enzyme is missing from the equation:

    ______ lipid+? à lipid-? Complexà monoglyceride (or glycerol)+fatty acids+?

    FILL IN THE BLANK
    Lipase
  27. Which enzyme is missing from the equation:

    ______starch + ? àstarch-?complexà maltose + ?  

    FILL IN THE BLANK
    Amalase
  28. Which enzyme is missing from the equation:

    ______ protein + ? -->protein- ? complex -->peptides+? 

    FILL IN THE BLANK
    Pepsin
  29. T or F: Buffering a solution has no effect on enzyme activity
    FALSE
  30. Which method is used to detect the presence of maltose?
    Benedict’s Test
  31. Which substance is needed for emulsification?
    Bile Salts
  32. E_______L_______ImmunoSorbent Assay

    FILL IN THE BLANK
    Enzyme Linked
  33. The ELISA we performed in lab for anti-HIV antibodies. Which procedure did we follow?
    Antigen--> serum--> antibody-linked Enzyme-->substrate    Anti-SALES
  34. What is an epitope?
    The specific site of an antigen that causes production of an antibody
  35. What is an antibody titer?

    FILL IN THE BLANK
    Measurement of how much antibody an organism has.
  36. What is the purpose of the enzyme that is linked to an antibody in an ELISA? 
    It catalyzes the conversation of a colorless substrate to a colored product.
  37. A Western Blot allows the separation of what? 
    Proteins
  38. ____ summation increases the number of motor units in muscle contraction. 

    FILL IN THE BLANK
    Spatial
  39. ____summation increases the rate of stimulus in muscle contractions?

    FILL IN THE BLANK
    Temporal
  40. T or F: A motor unit comprises of motor neuron and always more than one muscle fiber. 
    False
  41. What is the order of events at the neuromuscular junction? 
    Motor neuron action potentialàinflux of ca2 àrelease of AchàAction potential in muscle cell
  42. Which is not true of muscle fatigue?
    Depletion of Lactic acid
  43. A steady state of concentration when muscles are stimulated at high frequencies?
    Tetanization
  44. T or F: Muscles contract immediately after receiving a stimulus? 
    False
  45. What are the four events of a muscle twitch?
    STIMULUS, LATENT PERIOD, CONCENTRATION TIME, RELAXATION

    slap ctr

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